Webb Kristen M, Allard Marc W
Animal Parasitic Diseases Laboratory, Agricultural Research Service, United States Department of Agriculture Building 1180, Beltsville, MD 20705, USA.
J Forensic Sci. 2009 Mar;54(2):275-88. doi: 10.1111/j.1556-4029.2008.00952.x.
While the mitochondrial control region has proven successful for human forensic evaluations by indicating ethnic origin, domestic dogs (Canis lupus familiaris) of seemingly unrelated breeds often form large groups based on identical control region sequences. In an attempt to break up these large haplotype groups, we have analyzed the remaining c. 15,484 base pairs of the canine mitochondrial genome for 79 dogs and used phylogenetic and population genetic methods to search for additional variability in the form of single nucleotide polymorphisms (SNPs). We have identified 356 SNPs and 65 haplotypes in the remainder of the mitochondrial genome excluding the control region. The exclusion capacity was found to be 0.018. The mitochondrial control region was also evaluated for the same 79 dogs. The signals from the different fragments do not conflict, but instead support one another and provide a larger fragment of DNA that can be analyzed as forensic evidence.
虽然线粒体控制区已被证明通过指示种族起源在人类法医评估中很成功，但看似不相关品种的家犬（犬属狼种家犬）常常基于相同的控制区序列形成大的群体。为了打破这些大的单倍型群体，我们分析了79只狗的犬线粒体基因组剩余的约15484个碱基对，并使用系统发育和群体遗传学方法来寻找单核苷酸多态性（SNP）形式的额外变异性。我们在不包括控制区的线粒体基因组其余部分中鉴定出356个SNP和65个单倍型。排除能力为0.018。还对相同的79只狗评估了线粒体控制区。来自不同片段的信号并不冲突，而是相互支持，并提供了一个更大的可作为法医证据进行分析的DNA片段。
