National Institute of Criminalistics and Criminology, Vilvoordsesteenweg 100, B-1120 Brussels, Belgium; University of Antwerp (Evolutionary Ecology Group), Groenenborgerlaan 171, B-2020 Antwerp, Belgium.
University of Antwerp (Evolutionary Ecology Group), Groenenborgerlaan 171, B-2020 Antwerp, Belgium; Royal Belgian Institute of Natural Sciences (OD "Taxonomy and Phylogeny" and JEMU), Vautierstraat 29, B-1000 Brussels, Belgium.
Forensic Sci Int Genet. 2014 Sep;12:60-8. doi: 10.1016/j.fsigen.2014.05.001. Epub 2014 May 13.
A Belgian dog population sample and several population studies worldwide have confirmed that only a limited number of mtDNA control region haplotypes is observed in the majority of dogs. The high population frequency of these haplotypes negatively impacts both the exclusion probability of dog mtDNA analysis and the evidential value of a match with one of these haplotypes in casework. Variation within the mtDNA coding region was explored to improve the discrimination power of dog mtDNA analysis. In the current study, the entire mitochondrial genome of 161 dogs was sequenced applying a quality assured strategy and resulted in a total of 119 different mitochondrial genome sequences. Our research was focused on those dogs with the six most common control region haplotypes from a previous Belgian population study. We identified 33 informative SNPs that successfully divide the six most common control region haplotypes into 32 clusters of mitochondrial genome sequences. Determining the identity of these 33 polymorphic sites in addition to control region sequencing in case of a match with one of these 6 control region haplotypes could augment the exclusion probability of forensic dog mtDNA analysis from 92.5% to 97.5%.
一个比利时犬种群样本和全球多个种群研究证实，在大多数犬中只观察到有限数量的 mtDNA 控制区单倍型。这些单倍型的高种群频率对犬 mtDNA 分析的排除概率和在实际案例中与这些单倍型之一匹配的证据价值都产生了负面影响。为了提高犬 mtDNA 分析的判别能力，对 mtDNA 编码区的变异性进行了探索。在本研究中，应用质量保证策略对 161 只犬的整个线粒体基因组进行了测序，共获得了 119 个不同的线粒体基因组序列。我们的研究集中在之前的比利时种群研究中 6 种最常见的控制区单倍型的那些犬上。我们确定了 33 个信息性 SNP，这些 SNP 成功地将 6 种最常见的控制区单倍型分为 32 个线粒体基因组序列簇。在与这 6 种控制区单倍型之一匹配的情况下，除了控制区测序外，确定这 33 个多态性位点的身份可以将法医犬 mtDNA 分析的排除概率从 92.5%提高到 97.5%。
