Gopinath Subash Chandra Bose
Institute for Biological Resources and Functions & Center for Applied Near Field Optics Research (CAN-FOR), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba City 305-8562, Ibaraki, Japan.
Anal Chim Acta. 2009 Mar 23;636(2):117-28. doi: 10.1016/j.aca.2009.01.052. Epub 2009 Feb 4.
RNA-protein interactions are important biological events that perform multiple functions in all living organisms. The wide range of RNA interactions demands diverse conformations to provide contacts for the selective recognition of proteins. Various analytical procedures are presently available for quantitative analyses of RNA-protein complexes, but analytical-based mapping of these complexes is essential to probe specific interactions. In this overview, interactions of functional RNAs and RNA-aptamers with target proteins are discussed by means of mapping strategies.
RNA与蛋白质的相互作用是重要的生物学事件，在所有生物中发挥多种功能。广泛的RNA相互作用需要多样的构象来为蛋白质的选择性识别提供接触位点。目前有各种分析方法可用于RNA-蛋白质复合物的定量分析，但基于分析的这些复合物图谱绘制对于探究特定相互作用至关重要。在本综述中，通过图谱绘制策略讨论了功能性RNA和RNA适配体与靶蛋白的相互作用。
