Infectious Disease Cluster, Advanced Medical & Dental Institute, Universiti Sains Malaysia, 13200, Kepala Batas, Penang, Malaysia.
World J Microbiol Biotechnol. 2012 Jan;28(1):105-11. doi: 10.1007/s11274-011-0797-0. Epub 2011 Jun 3.
Purification of RNA fragments from a complex mixture is a very common technique, and requires consideration of the time, cost, purity and yield of the purified RNA fragments. This study describes the fastest method of purifying small RNA with the lowest cost possible, without compromizing the yield and purity. The technique describes the purification of small RNA from polyacrylamide gel, resulting in a good yield of small RNA with minimum experimental steps in avoiding degradation of the RNA, obviating the use of ethidium bromide and phenol-chloroform extraction, as well as siliconized glass wools to remove the polyacrylamide gel particles. The purified small RNA is suitable for a wide variety of applications such as ligation, end labelling with radio isotope, RT-PCR (Reverse Transcriptase-PCR), Northern blotting, experimental RNomics study and also Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
从复杂混合物中纯化 RNA 片段是一种非常常见的技术，需要考虑纯化的 RNA 片段的时间、成本、纯度和产量。本研究描述了一种最快的方法，可以在不影响产量和纯度的情况下，以最低的成本纯化小 RNA。该技术描述了从小型聚丙烯酰胺凝胶中纯化小 RNA，在避免 RNA 降解的情况下，以最少的实验步骤获得良好的小 RNA 产量，避免使用溴化乙锭和苯酚-氯仿提取，以及硅化玻璃棉去除聚丙烯酰胺凝胶颗粒。纯化的小 RNA 适用于多种应用，如连接、放射性同位素末端标记、RT-PCR（逆转录酶-PCR）、Northern 印迹、实验性 RNA 组学研究以及通过指数富集的配体系统进化（SELEX）。
