Deane D, Ueda N, Wise L M, Wood A R, Percival A, Jepson C, Inglis N F, Fleming S B, Mercer A A, McInnes C J
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, UK.
Virus Research Unit, University of Otago, Dunedin, New Zealand.
J Gen Virol. 2009 Apr;90(Pt 4):970-977. doi: 10.1099/vir.0.006692-0. Epub 2009 Mar 4.
The GIF protein of orf virus (ORFV) binds and inhibits the ovine cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). An equivalent protein has so far not been found in any of the other poxvirus genera and we therefore investigated whether it was conserved in the parapoxviruses. The corresponding genes from both the bovine-specific pseudocowpox virus (PCPV) and bovine papular stomatitis virus (BPSV) were cloned and sequenced. The predicted amino acid sequences of the PCPV and BPSV proteins shared 88 and 37 % identity, respectively, with the ORFV protein. Both retained the six cysteine residues and the WSXWS-like motif that are required for biological activity of the ORFV protein. However, an analysis of the biological activity of the two recombinant proteins revealed that, whilst the PCPV GIF protein bound to both ovine and bovine GM-CSF and IL-2 with very similar binding affinities to the ORFV GIF protein, no GM-CSF- or IL-2-binding activity was found for the BPSV protein.
口疮病毒（ORFV）的GIF蛋白可结合并抑制绵羊细胞因子粒细胞巨噬细胞集落刺激因子（GM-CSF）和白细胞介素-2（IL-2）。目前在其他痘病毒属中尚未发现类似蛋白，因此我们研究了其在副痘病毒中是否保守。克隆并测序了来自牛特异性假牛痘病毒（PCPV）和牛丘疹性口炎病毒（BPSV）的相应基因。PCPV和BPSV蛋白的预测氨基酸序列与ORFV蛋白的同源性分别为88%和37%。二者均保留了ORFV蛋白生物活性所需的六个半胱氨酸残基和WSXWS样基序。然而，对这两种重组蛋白生物活性的分析表明，虽然PCPV GIF蛋白与绵羊和牛GM-CSF以及IL-2的结合亲和力与ORFV GIF蛋白非常相似，但未发现BPSV蛋白具有GM-CSF或IL-2结合活性。
