Tohya Yukinobu, Narayanan Krishna, Kamitani Wataru, Huang Cheng, Lokugamage Kumari, Makino Shinji
Department of Microbiology and Immunology, The University of Texas Medical Branch at Galveston, Galveston, TX 77555-1019, USA.
J Virol. 2009 May;83(10):5282-8. doi: 10.1128/JVI.02485-08. Epub 2009 Mar 4.
nsp1 protein of severe acute respiratory syndrome coronavirus (SARS-CoV), a group 2b CoV, suppresses host gene expression by promoting host mRNA degradation and translation inhibition. The present study analyzed the activities of nsp1 proteins from the group 2 bat CoV strains Rm1, 133, and HKU9-1, belonging to groups 2b, 2c, and 2d, respectively. The host mRNA degradation and translational suppression activities of nsp1 of SARS-CoV and Rm1 nsp1 were similar and stronger than the activities of the nsp1 proteins of 133 and HKU9-1. Rm1 nsp1 expression in trans strongly inhibited the induction of type I interferon (IFN-I) and IFN-stimulated genes in cells infected with an IFN-inducing SARS-CoV mutant, while 133 and HKU9-1 nsp1 proteins had relatively moderate IFN-inhibitory activities. The results of our studies suggested a conserved function among nsp1 proteins of SARS-CoV and group 2 bat CoVs.
严重急性呼吸综合征冠状病毒（SARS-CoV，一种2b组冠状病毒）的nsp1蛋白通过促进宿主mRNA降解和抑制翻译来抑制宿主基因表达。本研究分析了分别属于2b、2c和2d组的2组蝙蝠冠状病毒株Rm1、133和HKU9-1的nsp1蛋白的活性。SARS-CoV的nsp1和Rm1 nsp1的宿主mRNA降解和翻译抑制活性相似，且强于133和HKU9-1的nsp1蛋白的活性。Rm1 nsp1的反式表达强烈抑制了感染诱导IFN的SARS-CoV突变体的细胞中I型干扰素（IFN-I）和IFN刺激基因的诱导，而133和HKU9-1的nsp1蛋白具有相对中等的IFN抑制活性。我们的研究结果表明SARS-CoV和2组蝙蝠冠状病毒的nsp1蛋白具有保守功能。
