[大肠杆菌RecA蛋白对经DNA修复抑制剂咖啡因和3-氨基苯甲酰胺处理后γ射线照射的HeLa细胞存活率的恢复作用]
[The restoration by Escherichia coli RecA protein of the survival of gamma-irradiated HeLa cells reduced by the DNA repair inhibitors caffeine and 3-aminobenzamide].
作者信息
Spivak I M, Kostetskiĭ I E, Shpilevaia S P, Kordium V A, Zhestianikov V D
出版信息
Tsitologiia. 1991;33(2):103-9.
It is confirmed that inhibitors of DNA repair caffeine and 3-aminobenzamide decrease the survival of gamma-irradiated HeLa cells. It is shown that the decreased survival of irradiated cells is reversed when Escherichia coli RecA protein is introduced into cell nucleases with the aid of liposomes. This effect is more expressed in caffeine-treated (before or after irradiation) than in 3-aminobenzamide-treated (before irradiation) cells. It is suggested that E. coli 38 kD RecA protein may compensate the function of HeLa RecA-like protein, inhibited by DNA repair inhibitors, which is necessary for the repair of single-strand breaks and double-strand breaks of DNA.
已证实，DNA修复抑制剂咖啡因和3 - 氨基苯甲酰胺会降低经γ射线照射的HeLa细胞的存活率。结果表明，借助脂质体将大肠杆菌RecA蛋白导入细胞核酶后，受照射细胞存活率的降低得以逆转。这种效应在咖啡因处理的细胞（照射前或照射后）中比在3 - 氨基苯甲酰胺处理的细胞（照射前）中更为明显。有人提出，大肠杆菌38 kD RecA蛋白可能补偿了被DNA修复抑制剂抑制的HeLa类RecA蛋白的功能，而该功能对于DNA单链断裂和双链断裂的修复是必需的。
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