Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium.
Br J Nutr. 2009 Sep;102(5):694-702. doi: 10.1017/S0007114509288982. Epub 2009 Mar 9.
The effect of dietary oligofructose and inulin supplementation on glucose metabolism in obese and non-obese cats was assessed. Two diets were tested in a crossover design; a control diet high in protein (46 % on DM basis), moderate in fat (15 %), low in carbohydrates (27 %), but no soluble fibres added; and a prebiotic diet, with 2.5 % of a mixture of oligofructose and inulin added to the control diet. Eight non-obese and eight obese cats were allotted to each of two diets in random order at intervals of 4 weeks. At the end of each testing period, intravenous glucose tolerance tests were performed. Area under the glucose curve (AUCgluc) was increased (P = 0.022) and the second insulin peak was delayed (P = 0.009) in obese compared to non-obese cats. Diets did not affect fasting plasma glucose concentrations, blood glucose response at each glucose time-point after glucose administration, AUCgluc, fasting serum insulin concentrations, area under the insulin curve, and height and appearance time of insulin response. Yet, analysis of acylcarnitines revealed higher propionylcarnitine concentrations (P = 0.03) when fed the prebiotic diet, suggesting colonic fermentation and propionate absorption. Prebiotic supplementation reduced methylmalonylcarnitine (P = 0.072) and aspartate aminotransferase concentrations (P = 0.025), both indicating reduced gluconeogenesis from amino acids. This trial evidenced impaired glucose tolerance and altered insulin response to glucose administration in obese compared to non-obese cats, regardless of dietary intervention; yet modulation of glucose metabolism by enhancing gluconeogenesis from propionate and inhibition of amino acid catabolism can be suggested.
研究了低聚果糖和菊粉补充剂对肥胖和非肥胖猫葡萄糖代谢的影响。采用交叉设计对两种饮食进行了测试；一种是高蛋白（DM 基础上 46%）、中脂肪（15%）、低碳水化合物（27%）但不添加可溶性纤维的对照饮食；另一种是添加 2.5%低聚果糖和菊粉混合物的益生元饮食。将 8 只非肥胖猫和 8 只肥胖猫随机分配到两种饮食中，间隔 4 周。在每个测试期结束时，进行静脉葡萄糖耐量试验。与非肥胖猫相比，肥胖猫的葡萄糖曲线下面积（AUCgluc）增加（P = 0.022），第二胰岛素峰延迟（P = 0.009）。饮食不影响空腹血糖浓度、葡萄糖给药后每个时间点的血糖反应、AUCgluc、空腹血清胰岛素浓度、胰岛素曲线下面积以及胰岛素反应的高度和出现时间。然而，酰基辅酶 A 分析显示，当给予益生元饮食时，丙酰基辅酶 A 浓度更高（P = 0.03），表明结肠发酵和丙酸吸收。益生元补充剂降低了甲基丙二酰基辅酶 A（P = 0.072）和天冬氨酸氨基转移酶浓度（P = 0.025），均表明氨基酸来源的糖异生减少。这项试验表明，肥胖猫的葡萄糖耐量受损，胰岛素对葡萄糖的反应与非肥胖猫相比发生改变，无论饮食干预如何；然而，通过增强丙酸来源的糖异生和抑制氨基酸分解代谢来调节葡萄糖代谢是可以被证实的。
