Mekebri A, Blondina G J, Crane D B
California Department of Fish and Game and Moss Landing Marine Laboratory, Rancho Cordova, CA 95670, USA.
J Chromatogr A. 2009 Apr 10;1216(15):3147-55. doi: 10.1016/j.chroma.2009.01.095. Epub 2009 Feb 5.
A liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method has been developed and validated to identify and quantify trace levels of cyanotoxins or microcystins (MC) in water, bivalves and fish tissue with enhanced sensitivity and specificity. The method enables confirmation and quantification of six MCs (MC-LA, LF, LR, LW, RR and YR) with a single chromatographic run. The applied chromatography also allows determination of certain MC metabolites (Desmethyl-LR and -RR). By using LC-ESI-MS/MS in multiple reaction monitoring (MRM) mode, the limit of detection and quantitation for the microcystins studied, were determined to be between 0.2 and 1 pg on column (5:1 S/N ratio). These values are below the 2 pg detection limits found in the available literature.
已开发并验证了一种液相色谱 - 电喷雾电离串联质谱法（LC - ESI - MS/MS），用于以更高的灵敏度和特异性鉴定和定量水、双壳贝类和鱼类组织中的痕量蓝藻毒素或微囊藻毒素（MC）。该方法能够在一次色谱运行中确认和定量六种微囊藻毒素（MC - LA、LF、LR、LW、RR和YR）。所应用的色谱法还可测定某些微囊藻毒素代谢物（去甲基 - LR和 - RR）。通过在多反应监测（MRM）模式下使用LC - ESI - MS/MS，所研究的微囊藻毒素的检测限和定量限在柱上为0.2至1皮克（信噪比为5:1）。这些值低于现有文献中发现的2皮克检测限。
