Department of Chemistry, Shahid Bahonar University of Kerman, Kerman, Iran P.O. Box, 76169-133, Kerman, Iran.
Young Researchers Society, Shahid Bahonar University of Kerman, P.O. Box, 76175-133, Kerman, Iran.
Sci Rep. 2017 Sep 8;7(1):11019. doi: 10.1038/s41598-017-11080-9.
The focus of this study is investigating the performance of graphene oxide (GO) in the protective effect of olive oil on Bacillus thuringiensis (Bt) after being exposed to UV radiations. Biological pesticides Bt subsp. Kurstaki is one of the most important biological control agents. We compared the protective effect of two UV protectant; GO and olive oil and also the combination of both, on the stability of the formulation of Bt after exposure to UV radiations. Spore viability was measured for protective effect and bioassay test was performed on the formulations of Bt. The combination of GO and olive oil revealed the highest viabilities of 50.62% after 96 h exposure to UV radiation, while viabilities of free spore, olive oil formulation and GO formulation were 32.54%, 37.19%,and 45.20%, respectively. The mortality of irradiated combination formulation on second-instar larvae Ephestia Kuehniella was 68.89%, while the same parameter for free spore, olive oil formulation and GO formulation were 40%, 46.66%,and 56%, respectively.
本研究的重点是研究氧化石墨烯（GO）在橄榄油保护苏云金芽孢杆菌（Bt）免受紫外线辐射后的保护性能。生物农药苏云金芽孢杆菌亚种。库尔塔基是最重要的生物防治剂之一。我们比较了两种紫外线保护剂；GO 和橄榄油以及两者的组合对暴露于紫外线辐射后 Bt 制剂稳定性的保护作用。用保护效果测量孢子活力，并对 Bt 制剂进行生物测定试验。GO 和橄榄油的组合在暴露于紫外线辐射 96 小时后表现出最高的活力，为 50.62%，而游离孢子、橄榄油制剂和 GO 制剂的活力分别为 32.54%、37.19%和 45.20%。组合制剂对二龄幼虫黄粉虫的致死率为 68.89%，而游离孢子、橄榄油制剂和 GO 制剂的相同参数分别为 40%、46.66%和 56%。
