MacDonald R C, Rempas S P
J Chromatogr. 1977 Jan 21;131:157-68. doi: 10.1016/s0021-9673(00)80929-2.
Separation of common phospholipids can be effected by dry column chromatography on silica gel. The method involves packing the column with dry gel and developing it in solvent mixtures used for thin-layer chromatography of the same lipids. Solvent is allowed to migrate only to the end of the column; access to the bands of separated material is obtained by using columns with a removable glass front. RF values of lipids on development columns and those on thin-layer plates are nearly identical when the column is packed with thin-layer chromatography gel. Such columns, however, develop very slowly. Columns packed with fine silica gel designed for elution column chromatography develop very rapidly and yield separations that are still quite comparable to those obtainable from thin-layer plates. Such columns are convenient for the purification of phospholipids in amounts of 10 mg to about 10 g. Column design and construction are described in detail.
普通磷脂的分离可以通过在硅胶上进行干柱色谱法来实现。该方法包括用干凝胶填充柱子，并在用于相同脂质薄层色谱的溶剂混合物中展开。仅允许溶剂迁移到柱的末端；通过使用带有可移动玻璃前端的柱子来获取分离物质的条带。当柱子用薄层色谱凝胶填充时，展开柱上脂质的比移值与薄层板上脂质的比移值几乎相同。然而，这样的柱子展开非常缓慢。填充用于洗脱柱色谱的细硅胶的柱子展开非常迅速，并且得到的分离效果仍然与从薄层板上获得的分离效果相当。这样的柱子便于纯化10毫克至约10克量的磷脂。详细描述了柱子的设计和构造。
