乙型流感病毒M基因中起始-终止五核苷酸进行蛋白质表达所需的区域。
Region required for protein expression from the stop-start pentanucleotide in the M gene of influenza B virus.
作者信息
Hatta Masato, Kohlmeier Candice K, Hatta Yasuko, Ozawa Makoto, Kawaoka Yoshihiro
机构信息
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Wisconsin 53706, USA.
出版信息
J Virol. 2009 Jun;83(11):5939-42. doi: 10.1128/JVI.00180-09. Epub 2009 Mar 11.
Abstract
Segment 7 of influenza B virus encodes two proteins, M1 and BM2. BM2 is expressed from a stop-start pentanucleotide, in which the BM2 initiation codon overlaps with the M1 stop codon. Here, we demonstrate that 45 nucleotides of the 3' end of the M1 coding region, but not the 5' end of the BM2 coding region, are sufficient for the efficient expression of the downstream protein. Placing these 45 nucleotides and the stop-start pentanucleotide in between the coding sequences induced the expression of at least three noninfluenza proteins, suggesting the utility of this system for expressing multiple proteins from one mRNA.
摘要
乙型流感病毒的第7节段编码两种蛋白，即M1和BM2。BM2由一个起始-终止五核苷酸表达，其中BM2起始密码子与M1终止密码子重叠。在此，我们证明M1编码区3'端的45个核苷酸，而非BM2编码区的5'端，足以有效表达下游蛋白。将这45个核苷酸和起始-终止五核苷酸置于编码序列之间可诱导至少三种非流感蛋白的表达，表明该系统可用于从一个mRNA表达多种蛋白。
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