Young J A, Cheung K S, Lang D J
J Infect Dis. 1977 May;135(5):837-40. doi: 10.1093/infdis/135.5.837.
Female mice were artificially inseminated with a solution of sperm and cytomegalovirus (experimental) or sperm alone (control). Maternal infection was usually induced when virus accompanied sperm at insemination. Fertilization occurred in the presence of virus, and embryogenesis appeared normal on gross examination, although a slight reduction in the number of embryos was observed in the experimental group. Tissue cultures were prepared after gestation for 14 days from individual and pooled embryos. In one of 28 instances, virus was recovered from blind-passaged, experimental, embryonic fibroblasts. All control cultures were negative. This model may prove useful in further study of the pathogenesis of intrauterine cytomegalovirus infection.
给雌性小鼠人工授精，一组授精精子和巨细胞病毒溶液（实验组），另一组仅授精精子（对照组）。授精时若病毒与精子一同存在，通常会引发母体感染。在有病毒存在的情况下发生了受精，大体检查时胚胎发育看似正常，不过实验组的胚胎数量略有减少。妊娠14天后，从单个及合并的胚胎制备组织培养物。在28个样本中有1个样本，从经盲目传代的实验组胚胎成纤维细胞中分离出了病毒。所有对照培养物均为阴性。该模型可能对进一步研究宫内巨细胞病毒感染的发病机制有用。
