Neighbour P A, Fraser L R
Fertil Steril. 1978 Aug;30(2):216-22. doi: 10.1016/s0015-0282(16)43463-1.
Male mice were inoculated with murine cytomegalovirus (MCMV) to produce an acute generalized infection. Infections virus was recovered from both epididymal sperm and seminal vesicles as well as from uterine sperm collected from mated females, suggesting that MCMV might be transmitted sexually. Because the presence of virus in the ejaculate might affect the fertilization process, the effect of MCMV on the fertilization of mouse gametes and on subsequent embryonic development was studied in vitro. Although the fertilization rate was reduced when sperm were preincubated with infectious virus, this was also the case when heat-inactivated virus was used, leading to the conclusion that this effect was not due to a direct infectious interaction between virus and gametes. Subsequent embryonic development was normal, and there was no evidence of productive infection of the preimplantation embryo.
雄性小鼠接种鼠巨细胞病毒（MCMV）以引发急性全身性感染。从附睾精子、精囊以及从交配雌性收集的子宫内精子中均分离出感染性病毒，这表明MCMV可能通过性传播。由于精液中病毒的存在可能影响受精过程，因此在体外研究了MCMV对小鼠配子受精及后续胚胎发育的影响。尽管当精子与感染性病毒预孵育时受精率降低，但使用热灭活病毒时也是如此，由此得出结论，这种影响并非由于病毒与配子之间的直接感染性相互作用所致。随后的胚胎发育正常，且没有证据表明植入前胚胎发生了增殖性感染。
