用于测定昆虫病原线虫感染性的检测方法比较
Comparison of assays for the determination of entomogenous nematode infectivity.
作者信息
Sims S R, Downing A S, Pershing J C
出版信息
J Nematol. 1992 Jun;24(2):271-4.
Injection, contact, and soil assays were used to compare infectivity of Heterorhabditis bacteriophora strain HP88 and Steinernema carpocapsae strain All to final instar Galleria mellonella larvae. Under comparable assay conditions, H. bacteriophora produced less Galleria mortality and showed greater within-assay variability in infectivity than S. carpocapsae. Injection of individual S. carpocapsae or H. bacteriophora infective juveniles into Galleria indicated that a comparatively greater percentage of S. carpocapsae was capable of initiating infection. In addition to nematode species, other major components of variability in assay estimations of nematode infectivity were number of nematodes used in the assay, assay type, date of the assay, and possibly, Galleria age.
采用注射、接触和土壤试验，比较了嗜菌异小杆线虫HP88品系和小卷蛾斯氏线虫All品系对大蜡螟末龄幼虫的感染力。在可比的试验条件下，嗜菌异小杆线虫导致的大蜡螟死亡率较低，且与小卷蛾斯氏线虫相比，其在试验内的感染力变异性更大。将单个小卷蛾斯氏线虫或嗜菌异小杆线虫感染性幼虫注射到蜡螟体内，结果表明，能够引发感染的小卷蛾斯氏线虫的比例相对更高。除线虫种类外，线虫感染力试验估计中变异性的其他主要因素包括试验中使用的线虫数量、试验类型、试验日期，以及可能的蜡螟年龄。
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