昆虫病原线虫浓度对液氮冷冻保存期间存活率的影响。
Effect of Entomopathogenic Nematode Concentration on Survival during Cryopreservation in Liquid Nitrogen.
作者信息
Bai Cheng, Shapiro-Ilan David I, Gaugler Randy, Yi Shuxia
出版信息
J Nematol. 2004 Sep;36(3):281-4.
Entomopathogenic nematodes are used for biological control of insect pests. A method for improved cryopreservation of infective juvenile stage nematodes has been developed using Steinernema carpocapsae and Heterorhabditis bacteriophora. Optimum survival for both species was achieved with 12,000 infective juveniles/ml in glycerol and 7,500/ml in Ringer's solution. For S. carpocapsae, maximum survival also was observed with 60,000 infective juveniles/ml in glycerol and 25,000/ml in Ringer's solution. These concentrations resulted in 100% post-cryopreservation survival of S. carpocapsae and 100% retention of original virulence to Galleria mellonella larvae. This is the first report of achieving 100% survival of an entomopathogenic nematode after preservation in liquid nitrogen. Maximum survival of H. bacteriophora following cryopreservation was 87%.
昆虫病原线虫被用于害虫的生物防治。利用小卷蛾斯氏线虫和嗜菌异小杆线虫，已开发出一种改进的感染期幼虫线虫冷冻保存方法。两种线虫在甘油中每毫升12000条感染期幼虫以及在林格氏液中每毫升7500条感染期幼虫的条件下均实现了最佳存活率。对于小卷蛾斯氏线虫，在甘油中每毫升60000条感染期幼虫以及在林格氏液中每毫升25000条感染期幼虫的条件下也观察到了最高存活率。这些浓度使小卷蛾斯氏线虫冷冻保存后的存活率达到100%，并对大蜡螟幼虫保持100%的原始毒力。这是关于昆虫病原线虫在液氮保存后实现100%存活率的首次报道。嗜菌异小杆线虫冷冻保存后的最高存活率为87%。
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