Homøe Preben, Bjarnsholt Thomas, Wessman Marcus, Sørensen Hans Christian Florian, Johansen Helle Krogh
Department of Otolaryngology, Head and Neck Surgery, F 2071, Rigshospitalet, University Hospital of Copenhagen, University of Copenhagen, Blegdamsvej 9, 2100, Copenhagen, Denmark.
Eur Arch Otorhinolaryngol. 2009 Oct;266(10):1533-8. doi: 10.1007/s00405-009-0940-9. Epub 2009 Mar 13.
Biofilm may explain the recurrences and recalcitrant episodes of otorrhea in chronic suppurative otitis media (CSOM). This study investigates bacterial biofilm in Greenlanders with CSOM and chronic otitis media with effusion (COME). The study is partly blinded, prospective and retrospective. Six children with CSOM, four children with COME and ten adults with CSOM were included in this study. Cultures were obtained and examined by standard methods. Otorrhea or glue was collected from the children and smears were prepared. Middle ear mucosa biopsies were obtained from the adults. Smears and biopsies were analyzed with microscopy and peptide nucleic acid fluorescence in situ hybridization (PNA-FISH). Biofilm was confirmed in 83% of CSOM smears but in none of the COME smears. Mucosal biofilm was confirmed in 80% of the biopsies from adults with CSOM. This study provides direct morphological evidence of biofilm in samples from human CSOM. This may help to explain the microbiological mechanisms of the disease and alter the treatment strategy in the future.
生物膜可能解释了慢性化脓性中耳炎（CSOM）中耳溢液的复发和难治性发作。本研究调查了患有CSOM和慢性渗出性中耳炎（COME）的格陵兰人的细菌生物膜。该研究部分为盲法、前瞻性和回顾性研究。本研究纳入了6名患有CSOM的儿童、4名患有COME的儿童和10名患有CSOM的成年人。通过标准方法获取并检查培养物。从儿童中收集耳溢液或胶样物并制备涂片。从成年人中获取中耳黏膜活检组织。通过显微镜检查和肽核酸荧光原位杂交（PNA-FISH）分析涂片和活检组织。在83%的CSOM涂片样本中证实存在生物膜，但在COME涂片样本中均未发现。在80%的患有CSOM的成年人活检组织中证实存在黏膜生物膜。本研究为人类CSOM样本中的生物膜提供了直接的形态学证据。这可能有助于解释该疾病的微生物学机制，并在未来改变治疗策略。
