Institute of Mountain Hazards and Environment, Chinese Academy of Science, Chengdu, 610041, China.
Environ Monit Assess. 2010 Apr;163(1-4):303-12. doi: 10.1007/s10661-009-0835-7. Epub 2009 Mar 13.
The short-term responses of H(2)O(2)-depletion-related parameters in moss Hypnum plumaeforme to the combined stress induced by Pb and Ni were investigated. The results showed that the Pb and Ni stress induced dose-dependent accumulation of hydrogen peroxide (H(2)O(2)). The increase of peroxidase (POD) activity and decrease of ascorbate peroxidase (APX) activity were observed under the combined heavy metal application. The antioxidants, ascorbate (AsA) and proline content, increased significantly when the metals were applied together. The study indicated that the cell damage caused by Pb stress was higher than that caused by Ni stress, Pb and Ni had synergistic effect in inducing the oxidative stress in moss H. plumaeforme, especially under the combination of high concentration of Ni (0.1 and 1.0 mM) and Pb. Content of proline, H(2)O(2) and the activity of POD, all showed a dose-dependent increase under Pb and Ni stress, suggesting their practical value as biomarkers in moss biomonitoring, especially in the case of light pollution caused by heavy metals without the changes in the appearance of mosses.
研究了 Pb 和 Ni 复合胁迫下，叶附生藓（Hypnum plumaeforme）中与 H2O2 耗竭相关参数的短期响应。结果表明，Pb 和 Ni 胁迫诱导了 H2O2 的剂量依赖性积累。在重金属共同作用下，过氧化物酶（POD）活性增加，抗坏血酸过氧化物酶（APX）活性降低。抗氧化剂抗坏血酸（AsA）和脯氨酸含量在金属共同作用下显著增加。研究表明，Pb 胁迫引起的细胞损伤高于 Ni 胁迫引起的损伤，Pb 和 Ni 在诱导叶附生藓（H. plumaeforme）氧化胁迫方面具有协同作用，尤其是在高浓度 Ni（0.1 和 1.0 mM）和 Pb 共同存在的情况下。脯氨酸、H2O2 和 POD 活性在 Pb 和 Ni 胁迫下均呈剂量依赖性增加，表明它们作为苔藓生物监测生物标志物具有实际价值，特别是在重金属引起的光污染而苔藓外观没有变化的情况下。
