Im Hyungsoon, Lesuffleur Antoine, Lindquist Nathan C, Oh Sang-Hyun
Laboratory of Nanostructures and Biosensing, Department of Electrical and Computer Engineering, University of Minnesota, Twin Cities, 200 Union Street South East, Minneapolis, Minnesota 55455, USA.
Anal Chem. 2009 Apr 15;81(8):2854-9. doi: 10.1021/ac802276x.
We present nanohole arrays in a gold film integrated with a six-channel microfluidic chip for parallel measurements of molecular binding kinetics. Surface plasmon resonance effects in the nanohole arrays enable real-time, label-free measurements of molecular binding events in each channel, while adjacent negative reference channels can record measurement artifacts such as bulk solution index changes, temperature variations, or changing light absorption in the liquid. With the use of this platform, streptavidin-biotin specific binding kinetics are measured at various concentrations with negative controls. A high-density microarray of 252 biosensing pixels is also demonstrated with a packing density of 10(6) sensing elements/cm(2), which can potentially be coupled with a massively parallel array of microfluidic channels for protein microarray applications.
我们展示了一种集成在金膜中的纳米孔阵列，该阵列与一个六通道微流控芯片相结合，用于并行测量分子结合动力学。纳米孔阵列中的表面等离子体共振效应能够实时、无标记地测量每个通道中的分子结合事件，而相邻的负参考通道可以记录测量伪像，如本体溶液折射率变化、温度变化或液体中光吸收的变化。利用该平台，在有阴性对照的情况下，在不同浓度下测量了链霉亲和素-生物素的特异性结合动力学。还展示了一个由252个生物传感像素组成的高密度微阵列，其填充密度为10(6)个传感元件/cm(2)，这有可能与用于蛋白质微阵列应用的大规模并行微流控通道阵列相结合。
