Yang Jiun-Chan, Ji Jin, Hogle James M, Larson Dale N
Department of Biochemistry and Molecular Pharmacology, Harvard Medical School, 240 Longwood Avenue, Boston, Massachusetts 02115, USA.
Nano Lett. 2008 Sep;8(9):2718-24. doi: 10.1021/nl801043t. Epub 2008 Aug 19.
We describe a nanoplasmonic probing platform that exploits small-dimension (<or=20 microm (2)) ordered arrays of subwavelength holes for multiplexed, high spatial resolution, and real-time analysis on biorecognition events. Nanohole arrays are perforated on a super smooth gold surface (roughness rms < 2.7 A) attached on a fluoropolymer (FEP) substrate fabricated by a replica technique. The smooth surface of gold provides a superb environment for fabricating nanometer features and uniform immobilization of biomolecules. The refractive index matching between FEP and biological solutions contributes to approximately 20% improvement on the sensing performance. Spectral studies on a series of small-dimension nanohole arrays from 1 microm (2) to 20 microm (2) indicate that the plasmonic sensing sensitivity improves as the gold-solution contact area increases. Our results also demonstrate that nanohole arrays with a dimension as small as 1 microm (2) can be used to effectively detect biomolecular binding events and analyze the binding kinetics. The future scientific opportunities opened by this nanohole platform include highly multiplexed analysis of ligand interactions with membrane proteins on high quality supported lipid bilayers.
我们描述了一种纳米等离子体探测平台，该平台利用亚波长孔的小尺寸（≤20 微米²）有序阵列，对生物识别事件进行多重、高空间分辨率和实时分析。纳米孔阵列是在通过复制技术制造的附着在含氟聚合物（FEP）基板上的超光滑金表面（粗糙度均方根<2.7 Å）上穿孔而成。金的光滑表面为制造纳米级特征和生物分子的均匀固定提供了极佳的环境。FEP 与生物溶液之间的折射率匹配使传感性能提高了约 20%。对一系列从 1 微米²到 20 微米²的小尺寸纳米孔阵列的光谱研究表明，随着金-溶液接触面积的增加，等离子体传感灵敏度提高。我们的结果还表明，尺寸小至 1 微米²的纳米孔阵列可用于有效检测生物分子结合事件并分析结合动力学。这个纳米孔平台带来的未来科学机遇包括对配体与高质量支撑脂质双分子层上的膜蛋白相互作用进行高度多重分析。
