Unit of Clinical Immunology, Uppsala University and Uppsala University Hospital, SE-75185 Uppsala, Sweden.
Autoimmun Rev. 2009 Sep;9(1):58-61. doi: 10.1016/j.autrev.2009.03.005. Epub 2009 Mar 11.
To evaluate the clinical utility of a commercial immunoblot assay for the detection of myositis-specific autoantibodies.
Serum samples from 153 myositis patients and 77 disease controls were investigated. The commercial Euroline assay with seven autoantigens (Mi-2, Ku, PM-Scl, Jo-1, Pl-7, Pl-12 and SSA/Ro-52) was used according to the manufacturer s instructions, and supplemented with an anti-SRP strip. In a separate experiment analyses were performed at different temperatures. Results were recorded with densitometry.
Anti-Jo-1 was found in 18 myositis and one systemic sclerosis patient. Antibodies against Mi-2 were found in 5 myositis patients, and eleven myositis patients had antibodies against PM-Scl. Four myositis patients showed anti-Pl-7 reactivity, whereas no patients had antibodies against Pl-12. Anti-Ku antibodies were found in 4 myositis and 2 primary Sjögren's syndrome patients. Anti-SRP was found in 8 myositis patients as well as in two disease controls. Antibodies against SSA/Ro52 ranged between 23-62% in all groups except juvenile dermatomyositis patients. Most autoantibody reactivities were clearly positive, only 11% (14/127) were borderline positive. Higher assay temperature increased antibody reactivities.
Except for anti-SSA/Ro-52 and anti-Ku the antibody reactivities were rather myositis-specific, supporting the use of this immunoblot assay. However, assay validation needs to be determined against other methods.
评估一种商业免疫印迹分析用于检测肌炎特异性自身抗体的临床效用。
研究了 153 例肌炎患者和 77 例疾病对照者的血清样本。根据制造商的说明，使用了带有七种自身抗原（Mi-2、Ku、PM-Scl、Jo-1、Pl-7、Pl-12 和 SSA/Ro-52）的商业 Euroline 检测试剂盒，并补充了抗 SRP 条。在另一个实验中，在不同温度下进行了分析。结果以密度计记录。
在 18 例肌炎和 1 例系统性硬化症患者中发现了抗 Jo-1。在 5 例肌炎患者中发现了针对 Mi-2 的抗体，11 例肌炎患者有针对 PM-Scl 的抗体。4 例肌炎患者显示抗 Pl-7 反应性，而没有患者有针对 Pl-12 的抗体。4 例肌炎和 2 例原发性干燥综合征患者中发现了抗 Ku 抗体。8 例肌炎患者和 2 例疾病对照者中发现了抗 SRP 抗体。除了青少年皮肌炎患者外，所有组中抗 SSA/Ro52 的抗体反应率均在 23%-62%之间。大多数自身抗体反应明显为阳性，只有 11%（14/127）为边缘阳性。较高的检测温度增加了抗体反应性。
除了抗 SSA/Ro-52 和抗 Ku 外，抗体反应性相当特异，支持使用这种免疫印迹分析。然而，需要针对其他方法来确定检测的验证。
