实验性糖尿病对雌性大鼠外阴阴道念珠菌病发生及维持的影响。
Effect of experimental diabetes on the development and maintenance of vulvovaginal candidiasis in female rats.
作者信息
Carrara Márcia A, Bazotte Roberto B, Donatti Lucélia, Svidzinski Terezinha I E, Consolaro Márcia E L, Patussi Eliana V, Batista Márcia R
机构信息
Postgraduate Program in Clinical Analyses, State University of Maringá, Maringá, Brazil.
出版信息
Am J Obstet Gynecol. 2009 Jun;200(6):659.e1-4. doi: 10.1016/j.ajog.2009.01.011. Epub 2009 Mar 16.
OBJECTIVE
The purpose of this study was to develop an experimental model of diabetes in female rats and verify its influence on vulvovaginal candidiasis.
STUDY DESIGN
The animals were divided into control and diabetic groups. Diabetes was induced with the use of an intravenous solution of alloxan (42 mg/kg bodyweight). One week after confirmation of hyperglycemia, the inoculation of Candida albicans yeast, previously standardized from a vaginal isolate, in concentrations of about 5 x 10(8), was performed. Infection control was made through vaginal culture, Papanicolaou cytology, and scanning electron microscopy (SCEM).
RESULTS
The results pointed to different glycemias between the control (74.8 +/- 2.6) and experimental groups (543.1 +/- 12.1) and a significant bodyweight decrease (227.6 +/- 4.77 and 204 +/- 6.39, respectively). The positive infection was shown by culture, Papanicolaou test, and SCEM in the experimental group.
CONCLUSION
Diabetes mellitus causes hyperglycemia, which was favorable to the vaginal colonization and infection by C albicans.
目的
本研究旨在建立雌性大鼠糖尿病实验模型，并验证其对外阴阴道念珠菌病的影响。
研究设计
将动物分为对照组和糖尿病组。使用四氧嘧啶静脉溶液（42毫克/千克体重）诱导糖尿病。在确认高血糖一周后，接种先前从阴道分离株标准化的白色念珠菌酵母，浓度约为5×10⁸。通过阴道培养、巴氏细胞学检查和扫描电子显微镜（SCEM）进行感染控制。
结果
结果显示对照组（74.8±2.6）和实验组（543.1±12.1）血糖不同，且体重显著下降（分别为227.6±4.77和204±6.39）。实验组通过培养、巴氏试验和SCEM显示感染呈阳性。
结论
糖尿病导致高血糖，有利于白色念珠菌在阴道定植和感染。
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