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一种具有S-亚硝基谷胱甘肽还原酶活性的金黄色葡萄球菌新型硝基还原酶。

A novel nitroreductase of Staphylococcus aureus with S-nitrosoglutathione reductase activity.

作者信息

Tavares Ana Filipa N, Nobre Lígia S, Melo Ana M P, Saraiva Lígia M

机构信息

Instituto de Tecnologia Química e Biológica, UNL, Av. da República (EAN), 2780-157 Oeiras, Portugal.

出版信息

J Bacteriol. 2009 May;191(10):3403-6. doi: 10.1128/JB.00022-09. Epub 2009 Mar 13.

DOI:10.1128/JB.00022-09
PMID:19286809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2687171/
Abstract

In this report we show that inactivation of the putative nitroreductase SA0UHSC_00833 (ntrA) increases the sensitivity of Staphylococcus aureus to S-nitrosoglutathione (GSNO) and augments its resistance to nitrofurans. S. aureus NtrA is a bifunctional enzyme that exhibits nitroreductase and GSNO reductase activity. A phylogenetic analysis suggests that NtrA is a member of a novel family of nitroreductases that seems to play a dual role in vivo, promoting nitrofuran activation and protecting the cell against transnitrosylation.

摘要

在本报告中，我们表明推定的硝基还原酶SA0UHSC_00833（ntrA）失活会增加金黄色葡萄球菌对S-亚硝基谷胱甘肽（GSNO）的敏感性，并增强其对硝基呋喃的抗性。金黄色葡萄球菌NtrA是一种双功能酶，具有硝基还原酶和GSNO还原酶活性。系统发育分析表明，NtrA是一个新型硝基还原酶家族的成员，该家族似乎在体内发挥双重作用，促进硝基呋喃的活化并保护细胞免受转亚硝基化作用。

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