Kaplan D T, Davis E L
J Nematol. 1991 Oct;23(4):446-50.
An in vitro bioassay with a 96-well microtiter plate was used to study the effect of lectins on burrowing nematode penetration of citrus roots. In each well, one 4-mm root segment, excised from the zone of elongation of rough lemon roots, was buried in 0.88 g dry sand. Addition of a Radopholus citrophilus suspension containing ca. 300 nematodes in 50 mu1 test solution completely moistened the sand in each well. The technique assured uniform treatment concentration throughout the medium. Within 16-24 hours, burrowing nematodes penetrated citrus root pieces, primarily through the cut ends. The lectins (100 mug/ml) Concanavalin A (Con A), soybean agglutinin (SBA), wheat germ agglutinin (WGA), and Lotus tetragonolobus agglutinin (LOT) stimulated an increase in penetration of citrus root segments by Radopholus citrophilus. Concentrations as low as 12.5 mug/ml Con A, LOT, and WGA stimulated burrowing nematode penetration of citrus roots. Heat denaturation of the lectins reversed their effect on penetration; however, incubation of nematodes in lectin (25 mug/ml) with 25 mM competitive sugars did not. The reason for enhanced penetration associated with lectins is unclear.
使用96孔微量滴定板进行体外生物测定，以研究凝集素对穿孔线虫穿透柑橘根的影响。在每个孔中，将从粗柠檬根伸长区切下的一段4毫米长的根段埋入0.88克干沙中。向50微升测试溶液中加入含有约300条线虫的柑橘穿孔线虫悬浮液，使每个孔中的沙子完全湿润。该技术确保了整个培养基中处理浓度的均匀性。在16至24小时内，穿孔线虫主要通过切口端穿透柑橘根段。凝集素（100微克/毫升）伴刀豆球蛋白A（Con A）、大豆凝集素（SBA）、麦胚凝集素（WGA）和百脉根凝集素（LOT）刺激柑橘穿孔线虫对柑橘根段的穿透增加。低至12.5微克/毫升的Con A、LOT和WGA浓度刺激穿孔线虫穿透柑橘根。凝集素的热变性逆转了它们对穿透的影响；然而，将线虫与25毫摩尔竞争性糖一起在凝集素（25微克/毫升）中孵育则没有这种效果。与凝集素相关的穿透增强的原因尚不清楚。
