Wang Jun, Yu Yong
Department of Biosystems Engineering, Zhejiang University, 268 Kaixuan Road, Hangzhou 310 029, People's Republic of China.
Luminescence. 2009 Jul-Aug;24(4):209-12. doi: 10.1002/bio.1096.
Ultra-weak luminescent analysis is a new way to detect the irradiation dose and the vigour of irradiated wheat. Wheat grain and wheat flour were used in this research for ultra-weak luminescent analysis. The experimental data showed that the bioluminescence intensity of wheat grain sample was different with increasing storage time and increasing dose, and a similar trend appeared in the germination rates of irradiated wheat grain. It was found that the differences in bioluminescence intensities and germination rates of irradiated wheat grain at different doses and storage times were due to the effect of irraditation on the wheat embryo and self-repair during storage. As a result, ultra-weak luminescent analysis cannot be used to detect the irradiation dose of irradiated wheat, but it can be used to determine vigour. Experiments showed that the irradiation dose had a highly significant effect on the bioluminescence intensities of wheat flour when cane sugar was added.
超微弱发光分析是检测辐照小麦辐照剂量和活力的一种新方法。本研究采用小麦籽粒和小麦粉进行超微弱发光分析。实验数据表明,小麦籽粒样品的生物发光强度随储存时间延长和剂量增加而不同,辐照小麦籽粒的发芽率也呈现类似趋势。研究发现,不同剂量和储存时间下辐照小麦籽粒的生物发光强度和发芽率差异是由于辐照对小麦胚的影响以及储存期间的自我修复作用。因此,超微弱发光分析不能用于检测辐照小麦的辐照剂量,但可用于测定活力。实验表明,添加蔗糖时,辐照剂量对小麦粉的生物发光强度有极显著影响。