Worden R M, Subramanian R, Bly M J, Winter S, Aronson C L
Department of Chemical Engineering, Michigan State University, East Lansing 48824-1226.
Appl Biochem Biotechnol. 1991 Spring;28-29:267-75. doi: 10.1007/BF02922606.
Bacillus stearothermophilus BR219, a phenol-resistant thermophile, can convert phenol to the specialty chemical catechol. The growth kinetics of this organism were studied in batch, continuous, and immobilized-cell culture. Batch growth was insensitive to pH between 6.0 and 8.0, but little growth occurred at 5.5. In continuous culture on a dilute medium supplemented with 10 mM phenol, several steady states were achieved between dilution rates of 0.25 and 1.3 h-1. Phenol degradation was found to be uncoupled from growth. Immobilized cells grew rapidly in a rich medium, but cell viability plummeted following a switch to a dilute medium supplemented with 5 mM phenol.
嗜热脂肪芽孢杆菌BR219是一种耐酚嗜热菌，能将苯酚转化为特殊化学品儿茶酚。研究了该微生物在分批培养、连续培养和固定化细胞培养中的生长动力学。分批培养时，在pH值为6.0至8.0之间对生长不敏感，但在pH值为5.5时几乎不生长。在添加10 mM苯酚的稀释培养基上进行连续培养时，在稀释率为0.25至1.3 h-1之间达到了几个稳定状态。发现苯酚降解与生长解偶联。固定化细胞在丰富培养基中快速生长，但在切换到添加5 mM苯酚的稀释培养基后，细胞活力急剧下降。
