嗜热脂肪芽孢杆菌苯酚降解菌的分离及苯酚羟化酶的部分特性研究
Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase.
作者信息
Gurujeyalakshmi G, Oriel P
机构信息
Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.
出版信息
Appl Environ Microbiol. 1989 Feb;55(2):500-2. doi: 10.1128/aem.55.2.500-502.1989.
Abstract
Bacillus stearothermophilus BR219, isolated from river sediment, degraded phenol at levels to 15 mM at a rate of 0.85 mumol/h (4 x 10(6) cells). The solubilized phenol hydroxylase was NADH dependent, exhibited a 55 degrees C temperature optimum for activity, and was not inhibited by 0.5 mM phenol.
摘要
从河流沉积物中分离出的嗜热脂肪芽孢杆菌BR219，能以0.85微摩尔/小时（4×10⁶个细胞）的速率降解高达15毫摩尔浓度的苯酚。溶解的苯酚羟化酶依赖于NADH，活性的最适温度为55℃，且不受0.5毫摩尔苯酚的抑制。
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