Ducrot P, Joundi A, Diebold M D, Pluot M
Laboratoire Central d'Anatomie, Centre Hospitalier Régional et Universitaire, Hôpital Robert-Debré, Reims.
Arch Anat Cytol Pathol. 1991;39(3):88-93.
The aim of the study was to evaluate the feasibility and possible contribution of silver stained nucleolar organizer regions (AgNORs) to prognostic considerations, in a series of 55 supratentorial gliomas: eight grade II astrocytomas, twelve grade III astrocytomas, thirty grade IV astrocytomas, two glioblastomas, one anaplastic oligodendroglioma, one oligodendroglioma and one ependymoma. Silver NORs (AgNORs) were demonstrated according to the method of Crocker et al. A difference between AgNOR sizes in peritumor and tumor tissue is noted. The mean NOR numbers in the tumor areas range from 0.871 to 2.677, without overlap between peritumor gliosis and glial tumors. A comparative analysis reveals significant correlations between the mean NOR number per nucleus and histological grading. This technique can play a practical role in the diagnosis and grading of tumors sampled by stereotactic biopsies: a count higher than 0.8 is highly suggestive of malignancy. In addition, the distribution of NORs may be important: intratumoral heterogeneity expresses various degrees of transcriptional activity between different glial tumors of the same grade. This technique provides information about the biological behaviour of glial tumors supplementary to that obtained from growth fraction analysis.
本研究的目的是评估银染核仁组织区（AgNORs）在55例幕上胶质瘤预后考量中的可行性及可能的贡献，这些胶质瘤包括：8例二级星形细胞瘤、12例三级星形细胞瘤、30例四级星形细胞瘤、2例胶质母细胞瘤、1例间变性少突胶质细胞瘤、1例少突胶质细胞瘤和1例室管膜瘤。根据克罗克等人的方法显示银染核仁组织区（AgNORs）。观察到肿瘤周围组织和肿瘤组织中AgNOR大小存在差异。肿瘤区域的平均核仁组织区数量范围为0.871至2.677，肿瘤周围胶质增生与胶质肿瘤之间无重叠。比较分析显示每个细胞核的平均核仁组织区数量与组织学分级之间存在显著相关性。该技术在立体定向活检所取肿瘤的诊断和分级中可发挥实际作用：计数高于0.8高度提示恶性肿瘤。此外，核仁组织区的分布可能很重要：肿瘤内异质性在同一级别的不同胶质肿瘤之间表现出不同程度的转录活性。该技术提供了关于胶质肿瘤生物学行为的信息，是从生长分数分析中获得的信息的补充。
