Tokiyoshi K, Yoshimine T, Maruno M, Nakagawa H, Iwata Y, Kuroda R, Hayakawa T
Depertment of Neurosurgery, Center for Adult Diseases, Osaka, Japan.
No To Shinkei. 1992 Dec;44(12):1083-6.
Nucleolar organizer regions (NORs) correspond to the loops of DNA which encode the ribosomal RNA. Acid proteins related to NORs can be stained by the silver colloidal technique (AgNORs). Since the configurations of AgNORs may be related to the protein metabolism or the proliferative activity of the cell, we tried to evaluate the corelationship between the morphology of AgNOR and the histologic malignancy in astrocytic tumors. For the quantitative evaluation the histographic pattern of AgNORs was analysed. Twenty-seven surgical specimens of astrocytomas (astrocytoma; 7 Cases, anaplastic astrocytoma; 9 cases, glioblastoma; 11 cases) were examined. The average of the means of AgNOR count in astrocytoma, anaplastic astrocytoma, glioblastoma were 1.68, 1.85 and 2.76 respectively. The averages of standard deviations (S. D.) of AgNOR count were 0.87, 1.03 and 1.26, respectively. In those tumors, the AgNOR histograms were flattered and the means and S. D. increased significantly as the malignancy increased. We speculate that the increased number and variations of AgNOR count could be a reflection of phenotypic alterations of astrocytoma cells such as cellular anaplasia and pleomorphism.
核仁组织区（NORs）对应于编码核糖体RNA的DNA环。与NORs相关的酸性蛋白可用银胶体技术（AgNORs）染色。由于AgNORs的形态可能与蛋白质代谢或细胞的增殖活性有关，我们试图评估AgNOR的形态与星形细胞瘤组织学恶性程度之间的相关性。为了进行定量评估，分析了AgNORs的组织图像模式。检查了27例星形细胞瘤手术标本（星形细胞瘤7例，间变性星形细胞瘤9例，胶质母细胞瘤11例）。星形细胞瘤、间变性星形细胞瘤、胶质母细胞瘤中AgNOR计数平均值分别为1.68、1.85和2.76。AgNOR计数的标准差（S.D.）平均值分别为0.87、1.03和1.26。在这些肿瘤中，AgNOR直方图变平，且随着恶性程度增加，平均值和标准差显著增加。我们推测，AgNOR计数的增加和变化可能反映了星形细胞瘤细胞的表型改变，如细胞间变和多形性。
