CD90 在人原代细胞上的表达及细胞培养中对成纤维细胞污染的清除。
CD90 Expression on human primary cells and elimination of contaminating fibroblasts from cell cultures.
机构信息
Department of Cell Therapy, Lonza Walkersville, Inc., Walkersville, MD, 21793, USA,
出版信息
Cytotechnology. 2009 Jan;59(1):31-44. doi: 10.1007/s10616-009-9190-3. Epub 2009 Mar 19.
Abstract
Cluster Differentiation 90 (CD90) is a cell surface glycoprotein originally identified on mouse thymocytes. Although CD90 has been identified on a variety of stem cells and at varying levels in non-lymphoid tissues such as on fibroblasts, brain cells, and activated endothelial cells, the knowledge about the levels of CD90 expression on different cell types, including human primary cells, is limited. The goal of this study was to identify CD90 as a human primary cell biomarker and to develop an efficient and reliable method for eliminating unwanted or contaminating fibroblasts from human primary cell cultures suitable for research pursuant to cell based therapy technologies.
摘要
簇分化抗原 90（CD90）是一种细胞表面糖蛋白，最初在小鼠胸腺细胞上被鉴定出来。尽管 CD90 已在多种干细胞上被鉴定出来，并且在非淋巴组织中以不同的水平存在，如成纤维细胞、脑细胞和激活的内皮细胞，但关于其在不同细胞类型（包括人原代细胞）上的表达水平的知识是有限的。本研究的目的是鉴定 CD90 作为人原代细胞生物标志物，并开发一种高效可靠的方法，从适合基于细胞治疗技术的研究的人原代细胞培养物中去除不需要的或污染的成纤维细胞。
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