Tenaud I, Saiagh I, Dreno B
UTCG, CHU Nantes, Institut de Biologie, Nantes Cedex, France.
J Dermatolog Treat. 2009;20(2):90-3. doi: 10.1080/09546630802509089.
Zinc and manganese, which are used in vivo because of their healing properties, have been shown to modulate in vitro integrin expression and to enhance keratinocyte migration. In addition, at the clinical level, a dressing of keratinocytes suspended in a fibrin glue has been proposed for the treatment of chronic wounds.
To investigate whether the addition of trace elements to this dressing could modulate the migratory phenotype of keratinocytes via the modulation of integrin expression in a manner similar to an in vitro model and thus increase the healing properties of this dressing.
Keratinocytes were mixed with Tissucol and maintained in culture for 12 days in a medium either supplemented or not with zinc or manganese. Then, integrin expression was studied by immunohistochemistry on fibrin clot cryosections.
We observed a significant increase of alpha5beta1 with zinc compared to the control medium. Zinc also enhanced alphaVbeta6 expression and manganese alpha5beta1, alphaVbeta5 and alphaVbeta6 expression, however without reaching a significant level.
By modulating integrin expression, trace elements can improve the efficiency of a biological dressing made of keratinocytes in a fibrin glue matrix and, thus, it appears beneficial to add them to this biological dressing for the treatment of skin defects.
锌和锰因其愈合特性而在体内被使用，已被证明可调节体外整合素表达并增强角质形成细胞迁移。此外，在临床层面，已提出用悬浮于纤维蛋白胶中的角质形成细胞敷料来治疗慢性伤口。
研究向该敷料中添加微量元素是否能通过以类似于体外模型的方式调节整合素表达来调节角质形成细胞的迁移表型，从而增强该敷料的愈合特性。
将角质形成细胞与纤维蛋白胶混合，并在添加或不添加锌或锰的培养基中培养12天。然后，通过免疫组织化学在纤维蛋白凝块冰冻切片上研究整合素表达。
与对照培养基相比，我们观察到添加锌后α5β1显著增加。锌还增强了αVβ6的表达，锰增强了α5β1、αVβ5和αVβ6的表达，但未达到显著水平。
通过调节整合素表达，微量元素可提高由纤维蛋白胶基质中的角质形成细胞制成的生物敷料的效率，因此，将它们添加到这种用于治疗皮肤缺损的生物敷料中似乎有益。
