Tenaud I, Leroy S, Chebassier N, Dreno B
Laboratory of Immuno-Dermatology, INSERM U463, CHU Hôtel-Dieu, Nantes, France.
Exp Dermatol. 2000 Dec;9(6):407-16. doi: 10.1034/j.1600-0625.2000.009006407.x.
The migration of keratinocytes plays an important role in the re-epithelialization of cutaneous wounds. Zinc, copper and manganese are used in vivo for their healing properties and their mechanism of action is still only partially known. Thus, they have been shown both to promote keratinocyte proliferation and to modulate integrins expression. The aim of this study was to determine if trace elements induce an increase of the migration of keratinocytes and if this effect is related to the modulation of integrins. Two independent migration assays were used to study keratinocyte migration: the scratch assay using normal human keratinocytes and the modified Boyden chamber using HaCaT cells. Inhibition studies using function-blocking antibodies directed to alpha3, alpha6, alpha(v) and beta1 subunits were performed to investigate the modulator effect of trace elements on integrin function. In this way, zinc and copper gluconates increased alpha3, alpha(v) and beta1 function whereas manganese gluconate seems mainly able to modulate the function of alpha3 and beta1. The stimulating effect of these trace elements on keratinocyte migration does not appear related to alpha6 subunit. Thus, zinc, copper and manganese enhanced keratinocyte migration and one of the mechanisms was going through a modulation of integrin functions.
角质形成细胞的迁移在皮肤伤口的再上皮化过程中发挥着重要作用。锌、铜和锰因其愈合特性而被用于体内，但其作用机制仍仅部分为人所知。因此，它们已被证明既能促进角质形成细胞增殖，又能调节整合素的表达。本研究的目的是确定微量元素是否会诱导角质形成细胞迁移增加，以及这种效应是否与整合素的调节有关。使用了两种独立的迁移试验来研究角质形成细胞迁移：使用正常人角质形成细胞的划痕试验和使用HaCaT细胞的改良博伊登小室试验。使用针对α3、α6、α(v)和β1亚基的功能阻断抗体进行抑制研究，以研究微量元素对整合素功能的调节作用。通过这种方式，葡萄糖酸锌和葡萄糖酸铜增加了α3、α(v)和β1的功能，而葡萄糖酸锰似乎主要能够调节α3和β1的功能。这些微量元素对角质形成细胞迁移的刺激作用似乎与α6亚基无关。因此，锌、铜和锰增强了角质形成细胞迁移，其机制之一是通过调节整合素功能来实现的。
