Panichayupakaranant Pharkphoom, Sakunpak Apirak, Sakunphueak Athip
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
J Chromatogr Sci. 2009 Mar;47(3):197-200. doi: 10.1093/chromsci/47.3.197.
A reversed-phase high-performance liquid chromatographic method is described for the simultaneous determination of four anthraquinones: rhein, aloe-emodin, emodin, and chrysophanol in Senna alata leaves. The method involves the use of a TSK-gel ODS-80Tm column (5 microm, 4.6 x 150 mm) at 25 degrees C with the mixture of methanol and 2% aqueous acetic acid (70:30, v/v) as the mobile phase and detection at 254 nm. The parameters of linearity, precision, accuracy, and specificity of the method were evaluated. The recovery of the method is 100.3-100.5%, and linearity (r(2) > 0.9998) was obtained for all anthraquinones. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 5%) were also achieved. The solvent for extraction of anthraquinones from S. alata leaves was examined in order to increase the anthraquinone content of the leaf extract. It was found that a solution of 5% hydrochloric acid (v/v), 5% ferric chloride (w/v), and 15% water in methanol (v/v) was capable of increasing the anthraquinone content in the leaf extract up to 1.67% (w/w).
描述了一种反相高效液相色谱法，用于同时测定番泻叶中四种蒽醌：大黄酸、芦荟大黄素、大黄素和大黄酚。该方法使用TSK-gel ODS-80Tm柱（5微米，4.6×150毫米），在25℃下，以甲醇和2%乙酸水溶液（70:30，v/v）的混合物为流动相，并在254纳米处进行检测。评估了该方法的线性、精密度、准确度和特异性参数。该方法的回收率为100.3 - 100.5%，所有蒽醌均获得了线性关系（r(2) > 0.9998）。还实现了高度的特异性以及重复性和重现性（相对标准偏差值小于5%）。为了提高叶提取物中蒽醌的含量，对从番泻叶中提取蒽醌的溶剂进行了考察。发现5%盐酸（v/v）、5%氯化铁（w/v）和15%水的甲醇溶液（v/v）能够将叶提取物中的蒽醌含量提高至1.67%（w/w）。
