Morrell J M, Dalin A M, Rodriguez-Martinez H
Swedish University of Agricultural Sciences-SLU, Department of Clinical Sciences, Division of Reproduction, Box 7054, SE-75007 Uppsala, Sweden.
Equine Vet J. 2009 Jan;41(1):53-8. doi: 10.2746/042516408x322139.
A new, simpler, technique of colloidal centrifugation has recently been developed, designated single layer centrifugation (SLC). This technique requires evaluation by comparison with a density gradient for its ability to select the best quality spermatozoa and its practicality of use on studfarms.
To compare the effect of 2 methods of colloidal centrifugation, density gradient centrifugation and single layer centrifugation, on stallion sperm motility, yield and survival, using freshly collected extended stallion semen.
Aliquots of extended stallion semen from 10 stallions (38 ejaculates) were processed by the 2 methods of colloidal centrifugation. For both uncentrifuged and centrifuged samples, sperm yield was calculated and subjective sperm motility assessed over several days to provide an estimate of sperm survival. Some stored semen samples, held at 4 degrees C overnight, were also available for testing.
For fresh, extended semen, a similar recovery yield of motile spermatozoa was seen for the 2 methods of preparation for single layers and density gradients, respectively. Sperm motility and survival rate were significantly improved by colloidal centrifugation compared to unprocessed ejaculate, without any significant difference between methods (SLC vs. gradient). However, the yield was reduced by 18-20% when cold-stored semen was used for centrifugation compared to fresh semen; and more variation between ejaculates was observed than for fresh ejaculates. Again, sperm motility and sperm survival were improved in the centrifuged sperm preparations compared to stored, unprocessed ejaculates.
The 2 colloid centrifugation techniques produce equivalent sperm preparations in terms of sperm quality. However, the SLC method would be more practical and convenient for use in the field.
最近开发了一种新的、更简单的胶体离心技术，称为单层离心法（SLC）。该技术需要通过与密度梯度法进行比较来评估其选择最佳质量精子的能力以及在种马场使用的实用性。
使用新鲜采集的稀释种马精液，比较两种胶体离心法（密度梯度离心法和单层离心法）对种马精子活力、产量和存活率的影响。
从10匹种马采集的38份射精的稀释精液等分试样，采用两种胶体离心法进行处理。对于未离心和离心后的样本，计算精子产量，并在几天内主观评估精子活力，以估计精子存活率。还提供了一些在4℃下保存过夜的精液样本用于测试。
对于新鲜的稀释精液，单层和密度梯度两种制备方法的活动精子回收率相似。与未处理的射精相比，胶体离心法显著提高了精子活力和存活率，两种方法之间无显著差异（SLC与梯度法）。然而，与新鲜精液相比，使用冷藏精液进行离心时产量降低了18 - 20%；并且与新鲜射精相比，射精之间的差异更大。同样，与未处理的冷藏射精相比，离心后的精子制剂中的精子活力和精子存活率有所提高。
两种胶体离心技术在精子质量方面产生等效的精子制剂。然而，SLC方法在现场使用将更实用和方便。
