用 siRNA 沉默 SATB1 可抑制小细胞肺癌细胞的增殖和侵袭。
Silencing SATB1 with siRNA inhibits the proliferation and invasion of small cell lung cancer cells.
机构信息
Department of Thoracic Surgery, the First Affiliated Hospital of Liaoning Medical University, No. 2 People street, 121000, Jinzhou, Liaoning, People's Republic of China.
Department of Kidney diseases, the First Affiliated Hospital of Liaoning Medical University, 121000, Jinzhou, Liaoning, People's Republic of China.
出版信息
Cancer Cell Int. 2013 Feb 5;13(1):8. doi: 10.1186/1475-2867-13-8.
BACKGROUND
Small cell lung cancer (SCLC) is a special kind of lung cancers, lymph or blood metastasis of SCLC usually occurs in early stage. Studies in breast and colon cancer showed over expression of SATB1 could promote tumor cell growth and inhibit apoptosis. Therefore, we studied the expression of SATB1 in SCLC.
METHODS
The level of SATB1 was analyzed in SCLC tissues, metastatic lymphoid nodes and adjacent normal lung tissues by immunohistochemistry. Meanwhile, small interfering SATB1-targeting RNA was constructed and transfected into human SCLC cell line NCI-H446 to evaluate the effects of SATB1-siRNA on cell proliferation, invasion and apoptosis.
RESULT
SATB1 protein was overexpressed in SCLC tissues and metastasis lymphoid nodes compared with adjacent normal lung tissues. Compared with control group, SATB1-siRNA inhibits the proliferation and invasion of SCLC cells and induces SCLC cells apoptosis statistically (P<0.05) in vitro.
CONCLUSION
Our results suggest that SATB1 plays an important role in the metastasis of human SCLC cell.
背景
小细胞肺癌（SCLC）是一种特殊类型的肺癌，SCLC 的淋巴或血液转移通常发生在早期。在乳腺癌和结肠癌的研究中表明，SATB1 的过表达可促进肿瘤细胞生长并抑制细胞凋亡。因此，我们研究了 SATB1 在 SCLC 中的表达。
方法
通过免疫组织化学分析 SCLC 组织、转移淋巴结和相邻正常肺组织中的 SATB1 水平。同时，构建靶向 SATB1 的小干扰 RNA 并转染入人 SCLC 细胞系 NCI-H446 中，以评估 SATB1-siRNA 对细胞增殖、侵袭和凋亡的影响。
结果
SATB1 蛋白在 SCLC 组织和转移淋巴结中过度表达，与相邻正常肺组织相比。与对照组相比，SATB1-siRNA 在体外显著抑制 SCLC 细胞的增殖和侵袭，并诱导 SCLC 细胞凋亡（P<0.05）。
结论
我们的结果表明 SATB1 在人 SCLC 细胞的转移中起重要作用。
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