通过在土壤基质膜系统中进行活力染色和显微操作培养苛求菌。
Cultivation of fastidious bacteria by viability staining and micromanipulation in a soil substrate membrane system.
作者信息
Ferrari B C, Gillings M R
机构信息
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Randwick, NSW 2052, Australia.
出版信息
Appl Environ Microbiol. 2009 May;75(10):3352-4. doi: 10.1128/AEM.02407-08. Epub 2009 Mar 20.
Abstract
Soil substrate membrane systems allow for microcultivation of fastidious soil bacteria as mixed microbial communities. We isolated established microcolonies from these membranes by using fluorescence viability staining and micromanipulation. This approach facilitated the recovery of diverse, novel isolates, including the recalcitrant bacterium Leifsonia xyli, a plant pathogen that has never been isolated outside the host.
摘要
土壤基质膜系统能够对苛求性土壤细菌作为混合微生物群落进行微培养。我们通过荧光活力染色和显微操作从这些膜上分离出已形成的微菌落。这种方法有助于获得多样的新型分离株，包括顽固性细菌木糖利夫森氏菌，一种从未在宿主外分离出来的植物病原体。
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本文引用的文献
1
Microbiology: metagenomics.
Nature. 2008 Sep 25;455(7212):481-3. doi: 10.1038/455481a.
2
Cultivating previously uncultured soil bacteria using a soil substrate membrane system.
Nat Protoc. 2008;3(8):1261-9. doi: 10.1038/nprot.2008.102.
3
Cultivation of hard-to-culture subsurface mercury-resistant bacteria and discovery of new merA gene sequences.
Appl Environ Microbiol. 2008 Jun;74(12):3795-803. doi: 10.1128/AEM.00049-08. Epub 2008 Apr 25.
4
Isolation of novel bacteria, including a candidate division, from geothermal soils in New Zealand.
Environ Microbiol. 2008 Aug;10(8):2030-41. doi: 10.1111/j.1462-2920.2008.01621.x. Epub 2008 Apr 15.
5
Incubation of environmental samples in a diffusion chamber increases the diversity of recovered isolates.
Appl Environ Microbiol. 2007 Oct;73(20):6386-90. doi: 10.1128/AEM.01309-07. Epub 2007 Aug 24.
6
Assessment and interpretation of bacterial viability by using the LIVE/DEAD BacLight Kit in combination with flow cytometry.
Appl Environ Microbiol. 2007 May;73(10):3283-90. doi: 10.1128/AEM.02750-06. Epub 2007 Mar 23.
7
Dimethyl sulfoxide (DMSO) waste residues and municipal waste water odor by dimethyl sulfide (DMS): the north-east WPCP plant of Philadelphia.
Environ Sci Technol. 2006 Jan 1;40(1):202-7. doi: 10.1021/es051312a.
8
Catalyzed reporter deposition-fluorescence in situ hybridization allows for enrichment-independent detection of microcolony-forming soil bacteria.
Appl Environ Microbiol. 2006 Jan;72(1):918-22. doi: 10.1128/AEM.72.1.918-922.2006.
9
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Appl Environ Microbiol. 2005 Dec;71(12):8714-20. doi: 10.1128/AEM.71.12.8714-8720.2005.
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Mechanism and use of the commercially available viability stain, BacLight.
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