Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Nishi-ku, Fukuoka, Japan.
J Biomed Mater Res A. 2010 Mar 1;92(3):1123-30. doi: 10.1002/jbm.a.32313.
Development of cell-patterning techniques is a major challenge for the construction of functional tissues and organs in tissue engineering. Recent progress in surface chemistry has enabled spatial control of cell adhesion onto cultural substrates by varying hydrophilicity, for example, by using poly (ethylene glycol) (PEG). In the present study, we developed a novel cell-patterning procedure using PEG-modified magnetite particles (PEG-Mags) and magnetic force. Using an array-patterned magnet, PEG-Mags were magnetically patterned on the surface of a tissue culture dish. The resultant substrate surface consisted of two regions: the PEG-Mag surface that acts as a cell-resistant region and the native substrate surface that promotes cell adhesion. When human keratinocyte HaCaT cells were seeded onto the PEG-Mag-patterned surface, cells adhered only to the native substrate surface, resulting in cell-patterning on the tissue culture dish. The patterned PEG-Mags were then washed away to expose the native substrate surface, and thereafter, when mouse myoblast C2C12 cells were seeded to the dish, cells adhered to the exposed substrate surface, resulting in a patterned coculture of heterotypic cells. Moreover, it is worth noting that the magnetic force-based cell-patterning procedure is not limited by the property of cultural substrate surfaces, and that cell-patterning of mouse fibroblast NIH3T3 cells on a monolayer of HaCaT cells was successfully achieved using PEG-Mags and magnetic force. These results indicate that this procedure provides a novel concept for cell-patterning and may be useful for tissue engineering and cell biology.
细胞图案化技术的发展是组织工程中构建功能性组织和器官的主要挑战。表面化学的最新进展使得通过改变亲水性（例如使用聚乙二醇（PEG））能够实现细胞在培养底物上的空间附着控制。在本研究中，我们使用 PEG 修饰的磁铁颗粒（PEG-Mags）和磁力开发了一种新的细胞图案化程序。使用图案化的磁铁，PEG-Mags 被磁力图案化在组织培养皿的表面上。所得的基底表面由两个区域组成：作为细胞抗性区域的 PEG-Mag 表面和促进细胞附着的天然基底表面。当人角质形成细胞 HaCaT 细胞接种到 PEG-Mag 图案化表面上时，细胞仅附着在天然基底表面上，从而在组织培养皿上进行细胞图案化。然后将图案化的 PEG-Mags 洗掉以露出天然基底表面，此后，当将小鼠成肌细胞 C2C12 细胞接种到培养皿上时，细胞附着在暴露的基底表面上，从而实现异质细胞的图案化共培养。此外，值得注意的是，基于磁力的细胞图案化程序不受培养基底表面性质的限制，并且使用 PEG-Mags 和磁力成功地实现了小鼠成纤维细胞 NIH3T3 细胞在单层 HaCaT 细胞上的细胞图案化。这些结果表明，该程序为细胞图案化提供了一个新的概念，可能对组织工程和细胞生物学有用。
