Animal Industry Division, Livestock Research Institute, Council of Agriculture, Tainan 712, Taiwan.
Evid Based Complement Alternat Med. 2011;2011:238208. doi: 10.1093/ecam/nep021. Epub 2010 Oct 25.
The purpose of the present study was to investigate the effects of Gingyo-san (GGS), a traditional Chinese medical formula, on peripheral lymphocyte proliferation and serum antibody titers in chickens vaccinated against the infectious bursal disease (IBD) virus. Treatment groups were fed one of three doses of GGS in their diet (0.5%, 1.0% and 2.0%, w/w), and the IBD vaccine was administered at 1 and 3 weeks of age. At Weeks 8, 12 and 16, changes in serum IBD antibody titers were measured via the micro-method and T cell proliferation. In gene expression experiments, GGS-treated peripheral T lymphocytes were stimulated with concanavalin A (ConA) for 24 h. The mRNA expression of interleukin-2 (IL-2), interferon-γ (IFN-γ), interleukin-4 (IL-4) and interleukin-12 (IL-12) was determined using a semi-quantitative RT-PCR assay. The results showed that a low dose of GGS could significantly raise the antibody titers. Medium and high doses of GGS enhanced IL-2 and IFN-γ production. GGS altered the expression of IL-4 and IL-12 in T lymphocytes. CD4(+) T lymphocyte development was also skewed towards the Th1 phenotype. GGS enhanced cell-mediated immunity and augmented the effects of IBD vaccination in strengthening subsequent anti-viral responses.
本研究旨在探讨金英散（GGS）对传染性法氏囊病（IBD）病毒疫苗接种鸡外周血淋巴细胞增殖和血清抗体效价的影响。治疗组分别以三种剂量（0.5%、1.0%和 2.0%，w/w）的 GGS 喂养，IBD 疫苗于 1 周龄和 3 周龄接种。在第 8、12 和 16 周，通过微量法和 T 细胞增殖测定血清 IBD 抗体效价的变化。在基因表达实验中，用刀豆蛋白 A（ConA）刺激 GGS 处理的外周 T 淋巴细胞 24 小时。使用半定量 RT-PCR 检测白细胞介素 2（IL-2）、干扰素-γ（IFN-γ）、白细胞介素 4（IL-4）和白细胞介素 12（IL-12）的 mRNA 表达。结果表明，低剂量 GGS 可显著提高抗体效价。中、高剂量的 GGS 增强了 IL-2 和 IFN-γ 的产生。GGS 改变了 T 淋巴细胞中 IL-4 和 IL-12 的表达。CD4(+)T 淋巴细胞的发育也向 Th1 表型倾斜。GGS 增强了细胞介导的免疫，增强了 IBD 疫苗接种的效果，增强了随后的抗病毒反应。
