Department of Life Sciences, National Chung Hsing University, No. 250, Kuo Kuang Road, Taichung 402, Taiwan.
Vaccine. 2010 Apr 1;28(16):2895-902. doi: 10.1016/j.vaccine.2010.01.066. Epub 2010 Feb 11.
The purpose of this study was to investigate the effects of dietary supplementation with recombinant porcine lactoferrin (rPLF) produced by yeast culture on peripheral lymphocyte proliferation and serum antibody titers in chickens vaccinated against the infectious bursal disease (IBD) virus. Treatment groups were fed with rPLF powder in their diet (2.0%, w/w), and the IBD vaccine was administrated at 1 and 3 weeks of age. At 8, 12, and 16 weeks after vaccination, serum IBD antibody titers were measured via the micro-method and T cell proliferation rates were evaluated. In gene expression analyses, rPLF-treated chicken peripheral T lymphocytes were stimulated with concanavalin A (ConA) for 24h. The mRNA expression levels of interleukin-2 (IL-2), interferon-gamma (IFN-gamma), interleukin-4 (IL-4), and interleukin-12 (IL-12) were determined using a semi-quantitative RT-PCR assay. The results revealed that the rPLF additive led to significant increases in serum IgG and IBD-specific antibody titers (P<0.05). The rPLF administration significantly increased chicken intestinal villous lengths and also enhanced the expression of IFN-gamma and IL-12 in chicken T lymphocytes. These data suggest that rPLF enhances cell-mediated immunity and augment the ability of IBD vaccination to benefit chicken industry in disease resistance.
本研究旨在探讨酵母培养生产的重组猪乳铁蛋白（rPLF）作为饲料添加剂对鸡外周血淋巴细胞增殖和血清抗体效价的影响。试验组鸡日粮中添加 rPLF （2.0%，w/w），于 1 日龄和 3 日龄接种传染性法氏囊病（IBD）疫苗。接种后 8、12 和 16 周，采用微量法测定血清 IBD 抗体效价，并用 ConA 刺激外周血 T 淋巴细胞，评价 T 细胞增殖率。在基因表达分析中，用 rPLF 处理鸡外周血 T 淋巴细胞 24h，采用半定量 RT-PCR 检测白细胞介素-2（IL-2）、干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）和白细胞介素-12（IL-12）的 mRNA 表达水平。结果表明，rPLF 添加剂显著提高了血清 IgG 和 IBD 特异性抗体效价（P<0.05）。rPLF 处理还显著增加了鸡肠绒毛长度，并增强了鸡 T 淋巴细胞中 IFN-γ和 IL-12 的表达。这些数据表明，rPLF 增强了细胞免疫，提高了 IBD 疫苗接种对鸡产业疾病抵抗力的有益作用。
