Institute of Vegetable Science, Zhejiang University, 268 Kaixuan Road, 310029, Hangzhou, China.
Mol Biol Rep. 2010 Feb;37(2):649-52. doi: 10.1007/s11033-009-9505-7. Epub 2009 Mar 24.
A bush-type plant was selected from tropical pumpkin 'cga' (Cucurbita moschata Duchesne) in order to study the vine development in C. moschata. In this study, a novel gene encoding NADH dehydrogenase was isolated from the vine line (cgaV) of C. moschata, that was not expressed in the near isogenic bush line (cgaBu). This gene, designated as CmV1 (C. moschata vine 1), was 545 bp in length and was composed of a 477 bp open reading frame, which had 99% nucleotide similarity to the chloroplast ndhJ gene for NADH dehydrogenase subunit J from Brassica oleracea. The deduced amino acid sequence of CmV1 had 99% similarity to NADH dehydrogenase subunit J from Arabidopsis and had 98% similarity to NADH dehydrogenase subunit from Barbarea verna. Analysis of the basic characteristics of the CmV1 protein revealed that it has one Respiratory chain NADH dehydrogenase 30 kD subunit signature, three N-myristoylation sites, one Casein kinase II phosphorylation site, and one Protein kinase C phosphorylation site. Reverse transcriptase-PCR analysis showed that CmV1 was expressed at a high level in the internodes and hypocotyls and was expressed stronger in elongating internodes than in fully expanded internodes. In conclusion, results obtained in the present study suggest that CmV1 gene might play important roles in vine elongation of tropical pumpkin.
为了研究南瓜蔓的生长发育，从南瓜品种‘cga’（ Cucurbita moschata Duchesne ）中选择了一种矮生型植株。在本研究中，从南瓜蔓（cgaV）中分离到一个编码 NADH 脱氢酶的新基因，该基因在近等基因的矮生型南瓜蔓（cgaBu）中不表达。该基因被命名为 CmV1（Cucurbita moschata vine 1），长度为 545bp，由一个 477bp 的开放阅读框组成，与拟南芥的叶绿体 ndhJ 基因编码 NADH 脱氢酶亚基 J 的核苷酸序列具有 99%的相似性。CmV1 的推导氨基酸序列与拟南芥的 NADH 脱氢酶亚基 J 具有 99%的相似性，与 Barbarea verna 的 NADH 脱氢酶亚基具有 98%的相似性。CmV1 蛋白的基本特征分析表明，它具有一个呼吸链 NADH 脱氢酶 30kD 亚基特征，三个 N-豆蔻酰化位点，一个酪蛋白激酶 II 磷酸化位点和一个蛋白激酶 C 磷酸化位点。反转录-PCR 分析表明，CmV1 在节间和下胚轴中高水平表达，在伸长节间中的表达强于完全展开的节间。综上所述，本研究结果表明，CmV1 基因可能在热带南瓜蔓的伸长发育中发挥重要作用。
