Department of Biological Science, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
Curr Opin Chem Biol. 2012 Apr;16(1-2):234-42. doi: 10.1016/j.cbpa.2011.12.007. Epub 2012 Jan 3.
Microarrays offer a compact solution for massively parallel screening. In recent years, microarrays have branched away from the exclusive pursuit of small molecule 'hits' in target centric screens, towards the sophisticated dissection of disease biology and comparative profiling of cellular states. This has led to innovative and instructive ways in which the platform may be deployed, providing new-found methods with which to harness the throughput achievable. Library design and diversity continues to drive success with peptide and small molecule microarrays. Newer synthesis and immobilization strategies extend the already wide repertoire of fabrication methods available. Herein we describe the latest advances in the small molecule and peptide microarray arena, which herald even more exciting breakthroughs in the coming decade.
微阵列为大规模并行筛选提供了一个紧凑的解决方案。近年来，微阵列已经不再仅仅追求针对特定靶点的小分子“命中物”，而是朝着对疾病生物学的复杂剖析和细胞状态的比较分析方向发展。这为平台的部署提供了创新和有指导意义的方法，为利用可实现的通量提供了新的方法。文库设计和多样性继续推动肽和小分子微阵列的成功。新的合成和固定化策略扩展了已经广泛可用的制造方法。本文介绍了小分子和肽微阵列领域的最新进展，预示着未来十年将有更多令人兴奋的突破。
