双链DNA的GC碱基对中中性鸟苷基自由基的单电子还原电位。
One-electron reduction potential of the neutral guanyl radical in the GC base pair of duplex DNA.
作者信息
Shinde Sujata S, Maroz Andrej, Hay Michael P, Anderson Robert F
机构信息
Department of Chemistry and Auckland Cancer Society Research Centre, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand.
出版信息
J Am Chem Soc. 2009 Apr 15;131(14):5203-7. doi: 10.1021/ja8087339.
Abstract
The one-electron oxidation of guanine in the GC base pair of DNA has been investigated using pulse radiolysis combined with DFT calculations. Reaction of benzotriazinyl radicals with DNA results in the formation of the neutral guanyl radical and redox equilibria. The one-electron reduction potential, E(7), of the neutral guanyl radical in the GC base pair is determined for the first time as 1.22 +/- 0.02 V, from both absorption and kinetic data.
摘要
利用脉冲辐解结合密度泛函理论计算，研究了DNA中鸟嘌呤-胞嘧啶（GC）碱基对中鸟嘌呤的单电子氧化过程。苯并三嗪基自由基与DNA反应会形成中性鸟苷基自由基和氧化还原平衡。根据吸收光谱和动力学数据，首次确定了GC碱基对中中性鸟苷基自由基的单电子还原电位E(7)为1.22±0.02 V。
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