Aljamali M N, Hern L, Kupfer D, Downard S, So S, Roe B A, Sauer J R, Essenberg R C
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, USA.
Insect Mol Biol. 2009 Apr;18(2):129-54. doi: 10.1111/j.1365-2583.2009.00863.x.
Ticks infest a wide range of hosts while bypassing their immune, inflammatory and haemostatic responses during their extended feeding, which may last for more than two weeks. Here, we present a transcriptome analysis of 3868 expressed sequence tags (ESTs) from three cDNA libraries generated from the salivary glands of adult female Ambyomma americanum ticks at different stages of feeding. We applied a normalization step for one library, significantly decreasing the abundance of mitochondrial sequences amongst the 2292 sequences from the normalized library. Our ESTs include homologues that may modulate haemostatic, immune and inflammatory responses of the hosts. Other ESTs probably represent important components of the highly efficient secretory pathways for salivary proteins and concomitantly transmitted pathogens.
蜱虫寄生于多种宿主，在长达两周多的长时间取食过程中，能避开宿主的免疫、炎症和止血反应。在此，我们展示了对成年雌性美洲钝缘蜱唾液腺在不同取食阶段生成的三个cDNA文库中3868个表达序列标签（EST）的转录组分析。我们对其中一个文库进行了标准化处理，显著降低了标准化文库中2292个序列中线粒体序列的丰度。我们的EST包括可能调节宿主止血、免疫和炎症反应的同源物。其他EST可能代表唾液蛋白和伴随传播病原体高效分泌途径的重要组成部分。
