Bohland Jason W, Wu Caizhi, Barbas Helen, Bokil Hemant, Bota Mihail, Breiter Hans C, Cline Hollis T, Doyle John C, Freed Peter J, Greenspan Ralph J, Haber Suzanne N, Hawrylycz Michael, Herrera Daniel G, Hilgetag Claus C, Huang Z Josh, Jones Allan, Jones Edward G, Karten Harvey J, Kleinfeld David, Kötter Rolf, Lester Henry A, Lin John M, Mensh Brett D, Mikula Shawn, Panksepp Jaak, Price Joseph L, Safdieh Joseph, Saper Clifford B, Schiff Nicholas D, Schmahmann Jeremy D, Stillman Bruce W, Svoboda Karel, Swanson Larry W, Toga Arthur W, Van Essen David C, Watson James D, Mitra Partha P
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
PLoS Comput Biol. 2009 Mar;5(3):e1000334. doi: 10.1371/journal.pcbi.1000334. Epub 2009 Mar 27.
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale of resolution suitable for comprehensive, brainwide coverage, using injections of tracers or viral vectors. We detail the scientific and medical rationale and briefly review existing knowledge and experimental techniques. We define a set of desiderata, including brainwide coverage; validated and extensible experimental techniques suitable for standardization and automation; centralized, open-access data repository; compatibility with existing resources; and tractability with current informatics technology. We discuss a hypothetical but tractable plan for mouse, additional efforts for the macaque, and technique development for human. We estimate that the mouse connectivity project could be completed within five years with a comparatively modest budget.
在这个全基因组时代，我们对神经解剖回路的了解仍然惊人地匮乏。然而，这些知识对于脑功能的基础研究和临床研究都至关重要。在此，我们倡导通过使用示踪剂或病毒载体注射，在适合全脑覆盖的介观分辨率尺度上对神经回路进行系统的实验性图谱绘制，来共同努力填补这一空白。我们详述了科学和医学依据，并简要回顾了现有知识和实验技术。我们定义了一系列要求，包括全脑覆盖；适用于标准化和自动化的经过验证且可扩展的实验技术；集中式、开放获取的数据存储库；与现有资源的兼容性；以及与当前信息技术的可处理性。我们讨论了一个针对小鼠的假设但可行的计划、猕猴的额外研究工作以及人类的技术开发。我们估计，小鼠连接性项目可以在相对适度的预算下于五年内完成。
