Wojciechowski Jason R, Shriver-Lake Lisa C, Yamaguchi Mariko Y, Füreder Erwin, Pieler Roland, Schamesberger Martin, Winder Christoph, Prall Hans Jürgen, Sonnleitner Max, Ligler Frances S
Naval Research Laboratory, 4555 Overlook Avenue SW, Washington, DC 20375, USA.
Anal Chem. 2009 May 1;81(9):3455-61. doi: 10.1021/ac8027323.
Biosensors have successfully demonstrated the capability to detect multiple pathogens simultaneously at very low levels. Miniaturization of biosensors is essential for use in the field or at the point of care. While microfluidic systems reduce the footprint for biochemical processing devices and electronic components are continually becoming smaller, optical components suitable for integration--such as LEDs and CMOS chips--are generally still too expensive for disposable components. This paper describes the integration of polymer diodes onto a biosensor chip to create a disposable device that includes both the detector and the sensing surface coated with immobilized capture antibody. We performed a chemiluminescence immunoassay on the OPD substrate and measured the results using a hand-held reader attached to a laptop computer. The miniaturized biosensor with the disposable slide including the organic photodiode detected Staphylococcal enterotoxin B at concentrations as low as 0.5 ng/mL.
生物传感器已成功证明能够在极低水平下同时检测多种病原体。生物传感器的小型化对于在现场或护理点使用至关重要。虽然微流体系统减小了生化处理设备的占地面积，并且电子元件不断变小，但适用于集成的光学元件（如发光二极管和互补金属氧化物半导体芯片）对于一次性元件来说通常仍然过于昂贵。本文描述了将聚合物二极管集成到生物传感器芯片上，以创建一种一次性设备，该设备包括检测器和涂有固定捕获抗体的传感表面。我们在邻苯二胺底物上进行了化学发光免疫测定，并使用连接到笔记本电脑的手持式阅读器测量结果。带有包括有机光电二极管的一次性载玻片的小型化生物传感器能够检测低至0.5 ng/mL浓度的葡萄球菌肠毒素B。
