Kodama A, Bauer S, Komatsu A, Asoh H, Ono S, Schmuki P
Department of Applied Chemistry, Kogakuin University, Tokyo, Japan.
Acta Biomater. 2009 Jul;5(6):2322-30. doi: 10.1016/j.actbio.2009.02.032. Epub 2009 Mar 4.
Apatite depositions from simulated body fluid (SBF) have been widely used for the in vitro assessment of the bioactivity of bone- and dental-implant materials. In previous work, we reported that titanium-based implant materials can be coated with an anodic TiO(2) nanotube layer which can significantly stimulate apatite formation. In the present work, we demonstrate that the tubular nature of such coatings makes them highly suitable for the application of a treatment called "alternative immersion method (AIM)", which preloads the coatings with synthetic hydroxyapatite. This treatment is indeed found to additionally promote natural apatite formation significantly. To study the AIM effect, layers of nanotubes with various diameters and crystal structures (amorphous, anatase/rutile) were produced, AIM-treated, and the formation of apatite in SBF10 (10mmol1(-1) HCO(3)(-)) was evaluated. The results show a drastic enhancement of apatite deposition rates (in some cases >20-fold acceleration) for AIM-treated TiO(2) nanotube layers in comparison with non-treated TiO(2) surfaces.
来自模拟体液（SBF）的磷灰石沉积已被广泛用于体外评估骨和牙种植材料的生物活性。在先前的工作中，我们报道了钛基种植材料可以涂覆阳极TiO(2)纳米管层，该层可以显著刺激磷灰石形成。在目前的工作中，我们证明了这种涂层的管状性质使其非常适合应用一种称为“交替浸泡法（AIM）”的处理方法，该方法用合成羟基磷灰石预加载涂层。实际上发现这种处理确实能显著促进天然磷灰石的形成。为了研究AIM效应，制备了具有不同直径和晶体结构（非晶态、锐钛矿/金红石）的纳米管层，进行AIM处理，并评估在SBF10（10mmol1(-1) HCO(3)(-)）中磷灰石的形成。结果表明，与未处理的TiO(2)表面相比，AIM处理的TiO(2)纳米管层的磷灰石沉积速率有显著提高（在某些情况下加速>20倍）。
