Byeon Seon-Mi, Kim Hye-Ji, Lee Min-Ho, Bae Tae-Sung
Department of Dental Biomaterials, Institute of Biodegradable Materials, School of Dentistry, Jeonbuk National University, Jeonju, Korea.
Korean J Orthod. 2022 Nov 25;52(6):412-419. doi: 10.4041/kjod22.118. Epub 2022 Aug 23.
This study evaluated the effect of cyclic pre-calcification treatment on the improvement of bioactivity and osseointegration of Ti-6Al-4V mini-screws.
The experimental groups were: an untreated group (UT), an anodized and heat-treated group (AH), and an anodized treatment followed by cyclic pre-calcification treatment group (ASPH). A bioactive material with calcium phosphate was coated on the mini-screws, and its effects on bioactivity and osseointegration were evaluated in in vitro and in vivo tests of following implantation in the rat tibia.
As a result of immersing the ASPH group in simulated body fluid for 2 days, protrusions appearing in the initial stage of hydroxyapatite precipitation were observed. On the 3rd day, the protrusions became denser, other protrusions overlapped and grew on it, and the calcium and phosphorus concentrations increased. The removal torque values increased significantly in the following order: UT group (2.08 ± 0.67 N·cm), AH group (4.10 ± 0.72 N·cm), and ASPH group (6.58 ± 0.66 N·cm) with the ASPH group showing the highest value (p < 0.05). In the ASPH group, new bone was observed that was connected to the threads, and it was confirmed that a bony bridge connected to the adjacent bone was formed.
In conclusion, it was found that the surface treatment method used in the ASPH group improved the bioactivity and osseointegration of Ti-6Al-4V orthodontic mini-screws.
本研究评估了循环预钙化处理对改善Ti-6Al-4V微型螺钉生物活性和骨整合的效果。
实验组包括：未处理组（UT）、阳极氧化和热处理组（AH）以及阳极氧化处理后进行循环预钙化处理组（ASPH）。在微型螺钉上涂覆含磷酸钙的生物活性材料，并在大鼠胫骨植入后的体外和体内试验中评估其对生物活性和骨整合的影响。
将ASPH组浸泡在模拟体液中2天后，观察到在羟基磷灰石沉淀初期出现突起。第3天，突起变得更密集，其他突起重叠并在其上生长，钙和磷浓度增加。去除扭矩值按以下顺序显著增加：UT组（2.08±0.67 N·cm）、AH组（4.10±0.72 N·cm）和ASPH组（6.58±0.66 N·cm），ASPH组的值最高（p<0.05）。在ASPH组中，观察到与螺纹相连的新骨，并且证实形成了与相邻骨相连的骨桥。
总之，发现ASPH组使用的表面处理方法改善了Ti-6Al-4V正畸微型螺钉的生物活性和骨整合。
