Douvaras Panagiotis, Antonatos Dionisios G, Kekou Kiriaki, Patsilinakos Sotirios, Chouliaras George, Christou Apostolos, Andrikou Anastasios, Kanavakis Emmanuel
Department of Medical Genetics, Athens University Medical School, Agia Sophia Children's Hospital, Athens, Greece.
Cardiology. 2009;114(1):11-8. doi: 10.1159/000210189. Epub 2009 Mar 31.
Vascular endothelial growth factor (VEGF) is upregulated in vivoin the ischemic human myocardium. Since several polymorphisms have been shown to influence VEGF expression, we evaluated the contribution of such polymorphisms to the clinical outcome of patients after an acute myocardial infarction (AMI).
PCR and restriction fragment length polymorphism analysis was performed to genotype 10 VEGF polymorphisms in 102 patients who had suffered an AMI and in 98 age- and sex-matched healthy individuals. Distribution of these polymorphisms was assessed by logistic regression analysis.
No significant differences were found between patients and normal individuals. However, when patients were subdivided into 2 groups based on the development of heart failure after their AMI judged by heart ultrasound measurements (ejection fraction <40%), the distribution of the -634 polymorphism differed significantly (p = 0.016). Specifically, patients with a CC genotype had 7 times higher risk of developing heart failure. Additionally, the co-inheritance of -634 with other VEGF polymorphisms was found to be significant for the development of heart failure between these 2 groups.
Our data indicate that the -634 polymorphism and its co-inheritance with genotypes of other VEGF polymorphisms might be considered as risk factors playing a role in the clinical outcome of AMI patients.
血管内皮生长因子（VEGF）在缺血的人体心肌中体内表达上调。由于已显示几种多态性会影响VEGF表达，我们评估了这些多态性对急性心肌梗死（AMI）患者临床结局的影响。
对102例发生AMI的患者和98例年龄及性别匹配的健康个体进行PCR和限制性片段长度多态性分析，以对10种VEGF多态性进行基因分型。通过逻辑回归分析评估这些多态性的分布。
患者与正常个体之间未发现显著差异。然而，当根据心脏超声测量（射血分数<40%）判断AMI后心力衰竭的发生情况将患者分为两组时，-634多态性的分布存在显著差异（p = 0.016）。具体而言，CC基因型患者发生心力衰竭的风险高7倍。此外，发现-634与其他VEGF多态性的共同遗传对于这两组之间心力衰竭的发生具有显著意义。
我们的数据表明，-634多态性及其与其他VEGF多态性基因型的共同遗传可能被视为在AMI患者临床结局中起作用的危险因素。
