Biochemistry and Molecular Biology Laboratory, Faculty of Pharmacy, Street Avicenne, 5019 Monastir, Tunisia.
Diagn Pathol. 2013 Jun 13;8:93. doi: 10.1186/1746-1596-8-93.
Myocardial infarction (MI) is a major clinical problem because of its large contribution to mortality. The genetic bases of this disease have been widely studied in recent years to find a clear association with some genetic markers that increase the risk of its occurrence. In the present investigation, the correlation between MI and the C3 complement polymorphism was analyzed using a case-control study.
Our study ported on one hundred seventy survived myocardial infarction patients and ninety five healthy controls. The C3 allele identification was investigated using the amplification refractory mutation system PCR to determine the C3S and the C3F alleles of the C3 polymorphism.
Frequencies of C3S and C3F in patients are 0.59 and 0.41 respectively. Fisher test results showed a significant increase of C3*F allele in the sample of patients (0.41; odds ratio: 2.616; C.I [1.738-3.938]) compared to controls (0.21; odds ratio: 0.382; 95% CI [0.254-0.575]), p = 2.742 × 10-6.
A strong positive correlation was found between C3 polymorphism and MI estimating that the risk of myocardial infarction is significantly increased among patients with C3*F allele of this polymorphism.
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1190484203893646.
心肌梗死（MI）是一个主要的临床问题，因为它对死亡率的影响很大。近年来，人们广泛研究了这种疾病的遗传基础，以发现与某些遗传标记的明确关联，这些遗传标记增加了其发生的风险。在本研究中，使用病例对照研究分析了 MI 与 C3 补体多态性的相关性。
我们的研究包括 170 名幸存的心肌梗死患者和 95 名健康对照者。使用扩增受阻突变系统 PCR 检测 C3 等位基因，以确定 C3 多态性的 C3S 和 C3F 等位基因。
患者中 C3S 和 C3F 的频率分别为 0.59 和 0.41。Fisher 检验结果显示，与对照组（0.21；比值比：0.382；95%CI[0.254-0.575]）相比，患者样本中 C3*F 等位基因的频率显著增加（0.41；比值比：2.616；95%CI[1.738-3.938]），p=2.742×10-6。
C3 多态性与 MI 之间存在很强的正相关性，估计该多态性的 C3*F 等位基因患者患心肌梗死的风险显著增加。
本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/1190484203893646。
