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本文引用的文献

1
Role of VEGF in organogenesis.VEGF 在器官发生中的作用。
Organogenesis. 2008 Oct;4(4):247-56. doi: 10.4161/org.4.4.7415.
2
VEGF and endothelial guidance in angiogenic sprouting.血管内皮生长因子与血管生成芽生中的血管内皮导向。
Organogenesis. 2008 Oct;4(4):241-6. doi: 10.4161/org.4.4.7414.
3
Association of VEGF gene polymorphisms with the development of heart failure in patients after myocardial infarction.心肌梗死后患者中VEGF基因多态性与心力衰竭发生的关联
Cardiology. 2009;114(1):11-8. doi: 10.1159/000210189. Epub 2009 Mar 31.
4
Mechanisms of single-stranded DNA-binding protein functioning in cellular DNA metabolism.单链DNA结合蛋白在细胞DNA代谢中的作用机制。
Biochemistry (Mosc). 2008 Dec;73(13):1388-404. doi: 10.1134/s0006297908130026.
5
Tissue-engineered blood vessels in pediatric cardiac surgery.小儿心脏手术中的组织工程血管
Yale J Biol Med. 2008 Dec;81(4):161-6.
6
Cardiac regeneration and stem cell therapy.心脏再生与干细胞治疗。
Curr Opin Organ Transplant. 2008 Oct;13(5):536-42. doi: 10.1097/MOT.0b013e32830fdfc4.
7
Guided stem cell cardiopoiesis: discovery and translation.引导性干细胞心脏生成：发现与转化。
J Mol Cell Cardiol. 2008 Oct;45(4):523-9. doi: 10.1016/j.yjmcc.2008.09.122. Epub 2008 Sep 26.
8
Ultrasound for drug and gene delivery to the brain.用于向大脑进行药物和基因递送的超声技术。
Adv Drug Deliv Rev. 2008 Jun 30;60(10):1209-17. doi: 10.1016/j.addr.2008.03.010. Epub 2008 Apr 6.
9
Ultrasonic gene and drug delivery to the cardiovascular system.超声介导的基因和药物向心血管系统的递送。
Adv Drug Deliv Rev. 2008 Jun 30;60(10):1177-92. doi: 10.1016/j.addr.2008.03.004. Epub 2008 Apr 3.
10
Stem cells used for cardiovascular tissue engineering.用于心血管组织工程的干细胞。
Eur J Cardiothorac Surg. 2008 Aug;34(2):242-7. doi: 10.1016/j.ejcts.2008.03.067. Epub 2008 May 8.

超声靶向微泡破坏介导pcDNA3.1⁻-hVEGF₁₆₅转染后间充质干细胞的蛋白表达

Protein expression of mesenchymal stem cells after transfection of pcDNA3.1⁻-hVEGF₁₆₅ by ultrasound-targeted microbubble destruction.

作者信息

Pu Zhaoxia, You Xiangdong, Xu Qiyuan, Gao Feng, Xie Xiaojie, Zhang Hong, Jian'an Wang

机构信息

Department of Ultrasound, Second Affiliated Hospital, Zhejiang University College of Medicine, 88 Jiefang Road, Hangzhou 310009, China.

出版信息

J Biomed Biotechnol. 2011;2011:839653. doi: 10.1155/2011/839653. Epub 2011 May 19.

DOI:10.1155/2011/839653
PMID:21716668
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3118296/
Abstract

Ultrasound-targeted microbubble destruction (UTMD) has been proposed as a new technique for organ-specific gene transfer and drug delivery. This study was performed to investigate the effect of UTMD on marrow mesenchymal stem cells (MSCs) transfected with pcDNA3.1⁻-hVEGF₁₆₅.pcDNA3.1⁻-hVEGF₁₆₅ were transfected into the third passage of MSCs, with or without UTMD under different ultrasound conditions. Protein expression was quantified by hVEGF₁₆₅-ELISA kit after transfection for 24, 48, and 72 hours. UTMD-mediated transfection of MSCs yielded a significant protein expression. UTMD of mechanic index (MI) 0.6 for 90 seconds led to the highest level of protein expression.

摘要

超声靶向微泡破坏（UTMD）已被提出作为一种用于器官特异性基因转移和药物递送的新技术。本研究旨在探讨UTMD对转染了pcDNA3.1⁻-hVEGF₁₆₅的骨髓间充质干细胞（MSC）的影响。将pcDNA3.1⁻-hVEGF₁₆₅转染至第三代MSC中，在不同超声条件下进行或不进行UTMD处理。转染24、48和72小时后，使用hVEGF₁₆₅-ELISA试剂盒对蛋白表达进行定量。UTMD介导的MSC转染产生了显著的蛋白表达。机械指数（MI）为0.6持续90秒的UTMD导致了最高水平的蛋白表达。